Effect of the method of conception and embryo transfer procedure on mid-gestation placenta and fetal development in an IVF mouse model
Effect of the method of conception and embryo transfer procedure on mid-gestation placenta and fetal development in an IVF mouse model
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DOI：
10.1093/humrep/deq165
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发表时间：
2010-08-01
影响因子：
6.1
通讯作者：
Rinaudo, P. F.
中科院分区：
文献类型：
--
作者：
Delle Piane, L.;Lin, W.;Rinaudo, P. F.
胎盘异常是解释体外受精后观察到的低出生体重发生率增加的潜在机制。本研究在小鼠模型中评估受孕和胚胎移植方法是否影响胎盘和胎儿发育。 IVF 囊胚 (CF1 x B6D2F1/J) 在 Whitten 培养基 (IVF(WM), n = 55) 或含有氨基酸的 K 改良单纯优化培养基 (IVF(KAA), n = 56) 中培养。胚胎被转移到假孕受体的子宫中。创建两个对照组：自然交配产生的未操作胚胎（体内组，n = 64）和自然交配产生的胚胎，从子宫冲洗并立即转移到假孕受体（冲洗囊胚，FB组，n = 57）。孕龄12.5天时，收集并固定植入部位；对胎儿和胎盘进行称重并评估其发育阶段（DS）。计算胎盘面积和血管体积分数；酌情应用参数统计。与 FB 小鼠相比，IVF 胎儿的发育出现适度但显着的延迟（P < 0.05）。此外，IVF 概念始终小于 FB (P < 0.05)。重要的是，在分析类似 DS 的胎儿时，这些差异仍然存在。 IVF 组的胎盘/胎儿比率 (IVF(WM) 0.95；IVF(KAA) = 0.90）大于 FB 组 (0.72)（所有比较 P < 0.05）。 IVF 组和 FB 组的胎盘大体形态和迷路/胎儿面积比率相当，胎儿血管、母体血液空间和滋养层成分的百分比也相同。体外胚胎培养影响胎儿和胎盘的发育；这可以解释体外受精后代出生体重较低的原因。
Abnormal placentation is a potential mechanism to explain the increased incidence of low birthweight observed after IVF. This study evaluates, in a mouse model, whether the method of conception and embryo transfer affect placentation and fetal development.IVF blastocysts (CF1 x B6D2F1/J) were cultured in Whitten's medium (IVF(WM), n = 55) or K modified simplex optimized medium with amino acids (IVF(KAA), n = 56). Embryos were transferred to the uteri of pseudo-pregnant recipients. Two control groups were created: unmanipulated embryos produced by natural mating (in vivo group, n = 64) and embryos produced by natural mating that were flushed from uterus and immediately transferred to pseudo-pregnant recipients (flushed blastocysts, FB group, n = 57). At gestation age 12.5 days, implantation sites were collected and fixed; fetuses and placentas were weighed and their developmental stage (DS) evaluated. Placental areas and vascular volume fractions were calculated; parametric statistics were applied as appropriate.IVF fetuses showed a modest but significant delay in development compared with FB mice (P < 0.05). In addition, IVF conceptuses were consistently smaller than FB (P < 0.05). Importantly, these differences persisted when analyzing fetuses of similar DS. The placenta/fetus ratio was larger in the IVF group (IVF(WM) 0.95; IVF(KAA) = 0.90) than the FB group (0.72) (P < 0.05 for all comparisons). Gross morphology of the placenta and ratio labyrinth/fetal area were equivalent in the IVF and FB groups, as were percentage of fetal blood vessels, maternal blood spaces and trophoblastic components.In vitro embryo culture affects fetal and placental development; this could explain the lower birthweight in IVF offspring.