Emergence of Clinical Pseudomonas aeruginosa Isolate Guangzhou-PaeC79 Carrying crpP, blaGES-5, and blaKPC-2 in Guangzhou of China.
Emergence of Clinical Pseudomonas aeruginosa Isolate Guangzhou-PaeC79 Carrying crpP, blaGES-5, and blaKPC-2 in Guangzhou of China.
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DOI：
10.1089/mdr.2020.0420
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发表时间：
2021-02
影响因子：
2.6
通讯作者：
Lin-Jing Wang;En-Zhong Chen;Ling Yang;Donghua Feng;Zhenbo Xu;Dingqiang Chen
中科院分区：
文献类型：
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作者：
Lin-Jing Wang;En-Zhong Chen;Ling Yang;Donghua Feng;Zhenbo Xu;Dingqiang Chen
铜绿假单胞菌作为一种潜在的“超级细菌”，仍然是抗生素耐药性的主要关注点。在这项研究中，发现临床铜绿假单胞菌分离株的出现在染色体上携带crpP和blaGES-5，在质粒上携带blaKPC-2。临床分离到一株广泛耐药铜绿假单胞菌Guangzhou-C79，对临床常用的所有抗生素均耐药。它含有一个染色体DNA和一个质粒，在染色体上鉴定出7个获得性抗菌素耐药基因，包括碳青霉烯耐药基因blaGES-5和氟喹诺酮耐药基因crpP，以及位于携带Tn 3样元件的IncP-6型质粒pPAEC 79上的碳青霉烯耐药基因blaKPC-2。以前从未报道过任何两种耐药基因的携带，同时携带三种bla和crpP可以解释细菌表型为对亚胺培南和美罗培南（≥16 μg/mL）的高水平耐药以及对环丙沙星和左氧氟沙星的耐药。
As a potential "Superbug," Pseudomonas aeruginosa remains the leading concern in antimicrobial resistance. In this study, the emergence of clinical P. aeruginosa isolate was found to carry crpP and blaGES-5 on the chromosome and blaKPC-2 on a plasmid. A clinical P. aeruginosa strain Guangzhou-PaeC79 with an extensively drug-resistant phenotype was isolated, which was resistant to all classes of clinical commonly used antibiotics. It contains one chromosomal DNA and one plasmid, with seven acquired antimicrobial resistance genes identified on the chromosome, including carbapenem resistance gene blaGES-5 and fluoroquinolone resistance gene crpP, and carbapenem resistance gene blaKPC-2 located on an IncP-6-type plasmid pPAEC79 carrying a Tn3-like element. Carriage of any two of the resistance genes has never been previously reported, and simultaneous carriage of three bla and crpP may explain the bacterial phenotype as high-level resistance to imipenem and meropenem (≥16 μg/mL) and resistance to ciprofloxacin and levofloxacin.