Intraspecific variation in the Egyptian scorpion Scorpio maurus palmatus venom collected from different biotopes
Intraspecific variation in the Egyptian scorpion Scorpio maurus palmatus venom collected from different biotopes
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DOI：
10.1016/j.toxicon.2008.12.007
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发表时间：
2009-03-01
期刊：
影响因子：
2.8
通讯作者：
McVean, Alistair
中科院分区：
文献类型：
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作者：
Abdel-Rahman, Mohamed A.;Omran, Mohamed Alaa A.;McVean, Alistair
本研究旨在探讨以下假设：（1）来自不同生境的蝎子（Scorpio maurus palmatus）的毒液是否具有种内多样性？(ii)如果是，这种变异是否与生态或遗传因素、地理距离和/或多个相互关联的参数有关？为了解决这些问题，从埃及四个地理上孤立的地方收集了蝎子。其中三个地点位于西奈半岛南部干旱生境中相互隔离的小块地区（瓦迪萨哈布、埃尔-阿格拉米亚和拉哈巴平原）。第四个群体是从西地中海海岸沙漠（WMCD）的半干旱生物区取样的。使用还原凝胶电泳（SDS-PAGE），我们已经显示了从这些不同地区收集的蝎子的肽表达的生物区特异性变化。WMCD来源的毒液样品含有比南西奈蝎毒液样品更高分子量的蛋白质组分（219、200、170、139、116 kDa）。南西奈毒液的特征在于存在11条蛋白质条带（93-0.58 kDa），这些条带在WMCD的单个毒液样品中没有反映出来。33和3.4 kDa的条带是所有蝎毒样品的特征，即使在西奈半岛南部地区，Sahab毒液也含有El-Agramia和Rahaba毒液样品中未检测到的五种组分。此外，雄性和雌性毒液分析揭示了一些与性别相关的蛋白质组的相似性和差异的蝎子种群。雌性毒液似乎比雄性毒液更复杂。雌性毒液样品显示219、200、77.5、55.5、45、39、37、24和16 kDa的条带，这些条带在雄性毒液中不存在。利用随机扩增多态DNA（RAPD）技术对4个蝎种群间的遗传距离进行了估计。RAPD分析结果证实了该群体在分子水平上的遗传多样性。从4个蝎种群中共扩增出77条RAPD带（大小在125 ~ 15，000 bp之间）。在77条谱带中，57条（76.2%）为多态谱带，20条为单态谱带。毒液和DNA的相似性系数数据被用来构建单独的树状图，其中分组在一起的西奈半岛南部的人口，这些是一些距离WMCD人口。综合以上分析，我们推测当地的环境条件、地理分离和遗传分离可能是导致中华绒螯蟹毒液种内变异的主要因素。M.掌骨(C)2008爱思唯尔有限公司保留所有权利。
The present study was conducted to explore the following hypotheses: (i) do scorpions (Scorpio maurus palmatus) from different biotopes exhibit intraspecific diversity in their venom? (ii) if so, is this variation associated with ecological or genetic factors, geographical distance, and/or multiple interrelated parameters? To address these questions, scorpions were collected from four geographically isolated localities in Egypt. Three of these locations are from mutually isolated pockets in the arid biotope of Southern Sinai (Wadi Sahab, El-Agramia and Rahaba plains). The fourth population was sampled from the semiarid biotope of Western Mediterranean Costal Desert (WMCD). Using reducing gel electrophoresis (SDS-PAGE), we have shown biotope-specific variation in the expression of peptides from scorpions collected from these distinct areas. WMCD sourced venom samples contain higher molecular weight protein components (219, 200,170,139,116 kDa) than Southern Sinai scorpion venom samples. The Southern Sinai venom is characterized by the presence of 11 protein bands (93-0.58 kDa) that are not mirrored in the individual venom samples of WMCD. Bands of 33 and 3.4 kDa were characteristics of all individual venom samples of the scorpion populations, Even within Southern Sinai area, Sahab venom contains five fractions that are not detected in both El-Agramia and Rahaba venom samples. Moreover, male and female venom analysis revealed some sex-related proteomic similarities and differences between scorpion populations. Female venom appears to be more complicated than the male venom. Female venom samples showed bands of 219, 200, 77.5, 55.5, 45, 39, 37, 24 and 16 kDa which were absent in the male venom. The random amplified polymorphic DNA (RAPD) technique was used to estimate the genetic distance between the four scorpion populations. The RAPD data confirmed the genetic diversity at molecular level among the sampled populations. More than 77 RAPD bands (ranging in size from 125 to 15,000 bp) were defined from the four scorpion populations. Of the 77 bands, 57 (76.2%) were polymorphic and 20 were monomorphic among the populations. The similarity coefficient data of venom and DNA were used to construct separate dendrograms, which grouped together the Southern Sinai populations and these were some distance away from the WMCD population. Taken together, we suspect that a combination of local environmental conditions, geographical separation and genetic separation may play a major role in the intraspecific variation of venom of S. m. palmatus. (C) 2008 Elsevier Ltd. All rights reserved.