High frequency of p53 gene alterations associated with protein overexpression in human squamous cell carcinoma of the larynx.
High frequency of p53 gene alterations associated with protein overexpression in human squamous cell carcinoma of the larynx.
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发表时间：
1992-06
期刊：
影响因子：
8
通讯作者：
R. Maestro;R. Dolcetti;D. Gasparotto;C. Doglioni;S. Pelucchi;L. Barzan;E. Grandi;M. Boiocchi
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文献类型：
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作者：
R. Maestro;R. Dolcetti;D. Gasparotto;C. Doglioni;S. Pelucchi;L. Barzan;E. Grandi;M. Boiocchi
通过免疫组织化学和分子相结合的方法，检测了一系列 58 个原发性人类喉鳞状细胞癌 (LSCC) 中 p53 肿瘤抑制基因的表达。 PAb1801、PAb122 和 PAb240 单克隆抗体免疫染色显示，约 60% 的病例显示核 p53 过度表达。这种现象与 p53 基因的结构和/或转录改变有关。我们的结果提供了证据，表明 p53 异常构成了迄今为止在 LSCC 中发现的最常见的遗传改变，并表明蛋白质的异常积累与 p53 突变版本的存在相关。这些发现与 p53 独特的生化特性相结合，支持了这样的假设：在这种肿瘤类型的发展过程中，接触烟雾致癌物与 p53 失活之间可能存在致病关系。
A series of 58 primary human squamous cell carcinomas of the larynx (LSCCs) was examined for the expression of the p53 tumor-suppressor gene by a combined immunohistochemical and molecular approach. About 60% of the cases displayed nuclear p53 overexpression as revealed by immunostaining with PAb1801, PAb122 and PAb240 monoclonal antibodies. This phenomenon was associated with the presence of structural and/or transcriptional alterations of the p53 gene. Our results provide evidence that p53 abnormalities constitute the most frequent genetic alteration identified so far in LSCC and indicate that the abnormal accumulation of the protein correlates with the presence of p53-mutated versions. These findings, taken together with the peculiar biochemical properties of p53, support the hypothesis of a possible pathogenetic relationship between smoke carcinogen exposure and p53 inactivation in the development of this tumor type.