The Design and Optimization of the PCR
The Design and Optimization of the PCR
复制标题
PCR的设计和优化
DOI：
10.1007/978-1-349-20235-5_1
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
R. Saiki
中科院分区：
文献类型：
--
作者：
R. Saiki
在引进以来的几年里，聚合酶链反应已经成为一种广泛的研究技术。就像PCR本身一样，其从业者的数量一直在呈指数级积累，并且随着该方法在分子生物学以外的领域得到更广泛的应用，在不久的将来可能会继续这样做。PCR的流行主要是由于其明显的简单性和高成功率。简化为最基本的术语，PCR仅涉及将DNA样品与寡核苷酸引物、脱氧核苷酸三磷酸和热稳定性Taq DNA聚合酶在合适的缓冲液中组合，然后重复加热和冷却混合物数小时，直到实现所需的扩增量。
In the few years since its introduction,1,2,3 the polymerase chain reaction has already become a widespread research technique. Like the PCR itself, the numbers of its practitioners have been accumulating exponentially and will probably continue to do so in the near future as the method finds wider applications in fields other than molecular biology. This popularity of the PCR is primarily due to its apparent simplicity and high probability of success. Reduced to its most basic terms, PCR merely involves combining a DNA sample with oligonucleotide primers, deoxynucleotide triphosphates, and the thermostable Taq DNA polymerase in a suitable buffer, then repetitively heating and cooling the mixture for several hours until the desired amount of amplification is achieved.
DOI：
10.1073/pnas.85.17.6252
发表时间：
1988-09-01
影响因子：
11.1
作者：
PETRUSKA, J;GOODMAN, MF;TINOCO, I
通讯作者：
TINOCO, I