Apoptosis-associated tyrosine kinase is a Cdk5 activator p35 binding protein
Apoptosis-associated tyrosine kinase is a Cdk5 activator p35 binding protein
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DOI：
10.1016/j.bbrc.2003.08.143
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发表时间：
2003-10-17
影响因子：
3.1
通讯作者：
Hisanaga, S
中科院分区：
文献类型：
--
作者：
Honma, N;Asada, A;Hisanaga, S
利用酵母双杂交系统，以CDK5激活子p35为诱饵，从人脑cDNA文库中筛选到人凋亡相关酪氨酸激酶(AATYK)的同源物KIAA0641的剪接变异体的3‘端片段。该克隆编码477个氨基酸，由KIAA0641内部的458个氨基酸组成，剪接后有19个氨基酸是该变异体所特有的，命名为hAATYKs-p35BP(hAATYK短异构体-p35结合多肽)。利用GST-融合蛋白，hAATYKs-p35BP可与大鼠脑提取液中的CDK5/p35结合。将KIAA0641的激动域与hAATYKs-p35BP的N端融合而成的hAATYK在HEK293细胞中与CDK5/p35结合。HAATYK和KIAA0641都与CDK5/p35结合并被CDK5/p35磷酸化。这些结果表明，hAATYK的两种异构体都是新的CDK5/p35结合蛋白和底物蛋白。(C)2003 Elsevier Inc.保留所有权利。
A 3'-terminal fragment of a splice variant of KIAA0641, a human homologue of apoptosis-associated tyrosine kinase (AATYK), was screened from human brain cDNA libraries by a yeast two-hybrid system using a Cdk5 activator p35 as a bait. The cloned cDNA encoded 477 amino acids, composed of internal 458 amino acids of KIAA0641 and 19 amino acids unique to this variant after splicing, then referred to this clone as hAATYKs-p35BP (human AATYK short isoform-p35 binding polypeptide). Using GST-fusion protein,hAATYKs-p35BP was shown to bind to Cdk5/p35 in a rat brain extract. hAATYKs made by fusing the kinase domain of KIAA0641 to the N-terminus of hAATYKs-p35BP was used for binding to Cdk5/p35 in HEK293 cells. Both hAATYKs and KIAA0641 bound to and were phosphorylated by Cdk5/p35. These results suggest that both isoforms of hAATYK are novel Cdk5/p35-binding and substrate proteins. (C) 2003 Elsevier Inc. All rights reserved.