Using bacteriophages to reduce formation of catheter-associated biofilms by Staphylococcus epidermidis
Using bacteriophages to reduce formation of catheter-associated biofilms by Staphylococcus epidermidis
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DOI：
10.1128/aac.50.4.1268-1275.2006
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发表时间：
2006-04-01
影响因子：
4.9
通讯作者：
Donlan, RM
中科院分区：
文献类型：
--
作者：
Curtin, JJ;Donlan, RM
由于生物膜的形成，留置导管的使用经常受到影响。本研究调查了用凝固酶阴性噬菌体预处理的水凝胶涂层导管是否会减少表皮葡萄球菌生物膜形成。生物膜上开发的水凝胶涂层的硅胶导管安装在一个修改的滴流反应器。用裂解S预处理导管段。通过在生物膜形成之前在37 ° C下将导管内腔暴露于10-log-PFU/ml噬菌体培养物1小时，来将导管内腔暴露于表皮噬菌体456。未处理的平均biotim细胞计数为7.01 +/- 0.47 log CFU/cm（2）导管。在有和没有补充二价阳离子的噬菌体处理下，log-CFU/cm（2）分别减少4.47（P < 0.0001）和2.34（P = 0.001）。二价阳离子补充而不使用噬菌体处理提供了0.67-log-CFU/cm（2）减少（P = 0.053）。用S.在体外模型系统中，表皮葡萄球菌噬菌体显著降低了S.在24小时的暴露期内，表皮的细菌，这表明噬菌体的潜力，减轻生物膜的形成留置导管和减少导管相关感染的发病率。
Use of indwelling catheters is often compromised as a result of biofilm formation. This study investigated if hydrogel-coated catheters pretreated with a coagulase-negative bacteriophage would reduce Staphylococcus epidermidis biofilm formation. Biofilms were developed on hydrogel-coated silicone catheters installed in a modified drip flow reactor. Catheter segments were pretreated with the lytic S. epidermidis bacteriophage 456 by exposing the catheter lumen to a 10-log-PFU/ml culture of the bacteriophage for 1 h at 37 degrees C prior to biofilm formation. The untreated mean biotilm cell count was 7.01 +/- 0.47 log CFU/cm(2) of catheter. Bacteriophage treatment with and without supplemental divalent cations resulted in log-CFU/cm(2) reductions of 4.47 (P < 0.0001) and 2.34 (P = 0.001), respectively. Divalent cation supplementation without bacteriophage treatment provided a 0.67-log-CFU/cm(2) reduction (P = 0.053). Treatment of hydrogel-coated silicone catheters with an S. epidermidis bacteriophage in an in vitro model system significantly reduced viable biofilm formation by S. epidermidis over a 24-h exposure period, suggesting the potential of bacteriophage for mitigating biofilm formation on indwelling catheters and reducing the incidence of catheter-related infections.