Detection and characterization of Wolbachia infections in laboratory and natural populations of different species of tsetse flies (genus Glossina).
Detection and characterization of Wolbachia infections in laboratory and natural populations of different species of tsetse flies (genus Glossina).
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DOI：
10.1186/1471-2180-12-s1-s3
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发表时间：
2012-01-18
期刊：
影响因子：
4.2
通讯作者：
Bourtzis K
中科院分区：
文献类型：
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作者：
Doudoumis V;Tsiamis G;Wamwiri F;Brelsfoard C;Alam U;Aksoy E;Dalaperas S;Abd-Alla A;Ouma J;Takac P;Aksoy S;Bourtzis K
沃尔巴克氏体是一种内共生α-变形菌属，感染广泛的节肢动物和丝虫线虫。Wolbachia能够诱导宿主细胞质不亲和性、雌体孤雌生殖、雌性化和雄性杀死等生殖异常，从而影响宿主的生物学、生态学和进化。该细菌群促进了关于其控制农业和医学疾病媒介的潜力的研究，包括Glossina spp.，它传播非洲锥虫，这是人类昏睡病和动物长角线虫病的病原体。在本研究中，我们采用了沃尔巴克氏体特异性的16 S rRNA PCR检测，以调查存在沃尔巴克氏体在6个不同的实验室股票以及在自然种群的9个不同的Glossina物种来自10个非洲国家。Wolbachia在Glossina morsitans morsitans、G. morsitans centralis和G.尼加拉瓜人。在G. brevipalpis，并首次在G. pallidipes和G.冈比亚须而在G. p. palpalis、毛果草G. fuscipes fuscipes和G.寄蝇使用16 S rRNA、wsp（Wolbachia表面蛋白）基因和MLST（多位点序列分型）基因标记对不同实验室和自然群体的Glossina物种的Wolbachia感染进行了表征。该分析导致检测到水平基因转移事件，其中Wobachia基因被插入采采蝇的核基因组中。沃尔巴克氏体感染在实验室和自然人群中检测到几个不同的Glossina物种。这些沃尔巴克氏体菌株的表征有望导致更深入地了解采采蝇-沃尔巴克氏体相互作用，这对于开发和使用基于沃尔巴克氏体的生物控制方法至关重要。
Wolbachia is a genus of endosymbiotic α-Proteobacteria infecting a wide range of arthropods and filarial nematodes. Wolbachia is able to induce reproductive abnormalities such as cytoplasmic incompatibility (CI), thelytokous parthenogenesis, feminization and male killing, thus affecting biology, ecology and evolution of its hosts. The bacterial group has prompted research regarding its potential for the control of agricultural and medical disease vectors, including Glossina spp., which transmits African trypanosomes, the causative agents of sleeping sickness in humans and nagana in animals. In the present study, we employed a Wolbachia specific 16S rRNA PCR assay to investigate the presence of Wolbachia in six different laboratory stocks as well as in natural populations of nine different Glossina species originating from 10 African countries. Wolbachia was prevalent in Glossina morsitans morsitans, G. morsitans centralis and G. austeni populations. It was also detected in G. brevipalpis, and, for the first time, in G. pallidipes and G. palpalis gambiensis. On the other hand, Wolbachia was not found in G. p. palpalis, G. fuscipes fuscipes and G. tachinoides. Wolbachia infections of different laboratory and natural populations of Glossina species were characterized using 16S rRNA, the wsp (Wolbachia Surface Protein) gene and MLST (Multi Locus Sequence Typing) gene markers. This analysis led to the detection of horizontal gene transfer events, in which Wobachia genes were inserted into the tsetse flies fly nuclear genome. Wolbachia infections were detected in both laboratory and natural populations of several different Glossina species. The characterization of these Wolbachia strains promises to lead to a deeper insight in tsetse flies-Wolbachia interactions, which is essential for the development and use of Wolbachia-based biological control methods.