Interleukin 15-dependent crosstalk between conventional and plasmacytoid dendritic cells is essential for CpG-induced immune activation
Interleukin 15-dependent crosstalk between conventional and plasmacytoid dendritic cells is essential for CpG-induced immune activation
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DOI：
10.1038/ni1348
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发表时间：
2006-07-01
影响因子：
30.5
通讯作者：
Ohteki, Toshiaki
中科院分区：
文献类型：
--
作者：
Kuwajima, Seiichi;Sato, Taku;Ohteki, Toshiaki
白细胞介素15（IL-15）在非甲基化CpG寡脱氧核苷酸（CpG）诱导的免疫应答中的作用尚不清楚。在这里，作为对CpG的反应，野生型和自然杀伤细胞耗尽的小鼠都产生了IL-12，并对致死剂量的单核细胞增生李斯特菌产生了抗性。相比之下，CpG处理的IL-15缺陷小鼠产生很少的IL-12，并死于L.单核细胞增多症CpG刺激的常规树突状细胞（cDCs）是IL-15和IL-12的主要产生者，但cDCs在不存在浆细胞样DCs（pDCs）的情况下不产生IL-12。cDC衍生的IL-15诱导cDC表达CD 40。cDC上的CD 40和pDC上的CD 40配体之间的相互作用导致cDC产生IL-12。因此，cDC和pDC之间的IL-15依赖性串扰对于CpG诱导的免疫活化是必需的。
The function of interleukin 15 (IL-15) in unmethylated CpG oligodeoxynucleotide (CpG)-induced immune responses remains unknown. Here, in response to CpG, both wild-type and natural killer cell-depleted mice produced IL-12 and became resistant to a lethal dose of Listeria monocytogenes. In contrast, CpG-treated IL-15-deficient mice produced little IL-12 and succumbed to L. monocytogenes. CpG-stimulated conventional dendritic cells (cDCs) were the main producers of both IL-15 and IL-12, but cDCs did not produce IL-12 in the absence of plasmacytoid DCs (pDCs). The cDC-derived IL-15 induced CD40 expression by cDCs. Interaction between CD40 on cDCs and CD40 ligand on pDCs led to IL-12 production by cDCs. Thus, IL-15-dependent crosstalk between cDCs and pDCs is essential for CpG-induced immune activation.