Characterization of a rhamnogalacturonan and a xyloglucan from Nerium indicum and their activities on PC12 pheochromocytoma cells
Characterization of a rhamnogalacturonan and a xyloglucan from Nerium indicum and their activities on PC12 pheochromocytoma cells
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DOI：
10.1021/np020118o
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发表时间：
2003-01-01
影响因子：
5.1
通讯作者：
Wu，HM
中科院分区：
文献类型：
--
作者：
Ding，K;Fang，JN;Wu，HM
从鼠李糖半乳葡聚糖（J1）和木葡聚糖（J2）中分离纯化得到两种多糖，并通过GC、GC - MS、NMR和ESMS对其进行了表征。鼠李糖半乳糖酸由结构为[→4)-α-GalpA(1→2)α-Rhap(1→4)-α-GalpA(1→4)−]n的线性三糖重复单元组成，而木葡聚糖则由结构为（1→4）-连接β-葡萄糖吡喃基残基的三糖重复单元组成，并取代在连接末端α-木吡喃糖侧链的6位上。两种多糖对PC12嗜铬细胞瘤细胞增殖和分化的影响与神经生长因子（NGF）相似。
Two polysaccharides, a rhamnogalacturonan (J1) and a xyloglucan (J2), were isolated and purified from the whole flowers ofNeriumindicumand characterized by GC, GC−MS, NMR, and ESMS. The rhamnogalacturonan was found to consist of repeating units of a linear trisaccharide with the structure [→4)-α-GalpA(1→2)α-Rhap(1→4)-α-GalpA(1→4)−]n, while the xyloglucan consisted of repeating units of the trisaccharide, which was (1→4)-linked β-glucopyranosyl residues as a backbone and substituted at position 6 linked to terminal α-xylopyranose side chains. These two polysaccharides were tested on the proliferation and differentiation on PC12 pheochromocytoma cells and found to have effects similar to nerve growth factor (NGF).