The application of titanium dioxide (TiO(2)) nanoparticles in the photo-thermal therapy of melanoma cancer model.
The application of titanium dioxide (TiO(2)) nanoparticles in the photo-thermal therapy of melanoma cancer model.
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DOI：
10.22038/ijbms.2018.30284.7304
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发表时间：
2018-11
影响因子：
2.2
通讯作者：
Dehghanian AR
中科院分区：
文献类型：
--
作者：
Behnam MA;Emami F;Sobhani Z;Dehghanian AR
光热疗法（PTT）是一种将光子能量转化为热能诱导恶性肿瘤细胞热疗的治疗方法。在该方法中，通过纳米颗粒（NPs）进行能量转换以提高诱导热效率。该技术中使用的NPs具有低细胞毒性和高光吸收性。在本研究中，二氧化钛（TiO2） NPs作为PTT剂。为了提高水的分散性和生物相容性，我们合成了聚乙二醇(PEG)-TiO2 NPs (PEGylated TiO2 NPs)，并实验评估了这些NPs对PTT后黑色素瘤肿瘤大小的影响。为了提高TiO2 NPs在水中的分散性，采用聚乙二醇包覆TiO2 NPs表面。通过热重分析和透射电镜技术证实了在TiO2 NPs周围形成了一层薄薄的PEG。将40只雌性癌变小鼠分为4组，每组1次给予NPs和激光二极管（λ = 808 nm, P = 2 W, I = 2 W/cm2）治疗7 min。与仅接受激光治疗的小鼠相比，接受激光激发的TiO2-PEG NPs治疗小鼠的平均肿瘤大小急剧下降。动物实验结果表明，聚乙二醇化的TiO2 NPs在PTT技术中对实体肿瘤的破坏非常有效。
Photo-thermal therapy (PTT) is a therapeutic method in which photon energy is converted into heat to induce hyperthermia in malignant tumor cells. In this method, energy conversion is performed by nanoparticles (NPs) to enhance induced heat efficacy. The low-cytotoxicity and high optical absorbance of NPs used in this technique are very important. In the present study, titanium dioxide (TiO2) NPs were used as agents for PTT. For increasing water dispersibility and biocompatibility, polyethylene glycol (PEG)-TiO2 NPs (PEGylated TiO2 NPs) were synthesized and the effect of these NPs on reducing melanoma tumor size after PTT was experimentally assessed. To improve the dispersibility of TiO2 NPs in water, PEG was used for wrapping the surface of TiO2 NPs. The formation of a thin layer of PEG around the TiO2 NPs was confirmed through thermo-gravimetric analysis and transmission electron microscopy techniques. Forty female cancerous mice were divided into four equal groups and received treatment with NPs and a laser diode (λ = 808 nm, P = 2 W & I = 2 W/cm2) for seven min once in the period of the treatment. Compared to the mice receiving only the laser therapy, the average tumor size in the mice receiving TiO2-PEG NPs with laser excitation treatment sharply decreased. The results of animal studies showed that PEGylated TiO2 NPs were exceptionally potent in destroying solid tumors in the PTT technique.