A stromal cell line from myeloid long-term bone marrow cultures can support myelopoiesis and B lymphopoiesis.
A stromal cell line from myeloid long-term bone marrow cultures can support myelopoiesis and B lymphopoiesis.
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DOI：
10.4049/jimmunol.138.4.1082
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发表时间：
1987-02
影响因子：
4.4
通讯作者：
L. Collins;K. Dorshkind
中科院分区：
文献类型：
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作者：
L. Collins;K. Dorshkind
B淋巴细胞和髓样细胞的产生发生在骨髓中，与基质细胞的支持群体相关。为了确定这些过程是否依赖于相同或不同的基质细胞群，从Dexter型长期骨髓培养物的粘附层产生基质细胞系。这些培养物支持髓样细胞及其前体、B细胞前体和粘附层细胞，支持B细胞在适当条件下分化。所检测的两个细胞系S10和S17表达I类组织相容性抗原，但不表达其他造血细胞表面决定簇，如Thy-1、Lyt-1、IG、Ia、Mac-1或BP-1。在Dexter条件下，两种细胞系在接种尼龙羊毛通过的骨髓后均可支持骨髓生成。尼龙毛通道消耗能够在体外形成粘附层的基质细胞，但保留造血前体。接种后3周恢复的非贴壁细胞群中的细胞和集落形成单位-粒细胞/巨噬细胞的数量分别增加了19倍和10倍，在S10和S17的restenosis培养物中。在Dexter条件下生长3周后，将恢复培养物转移到Whitlock和Witte描述的B细胞分化的最佳条件下。生长4周后，造血细胞从S17培养物中持续回收，但S10培养物中没有。来自S17培养物的这些细胞中的一部分表达14.8抗原并且是表面IgM阳性。S10培养物中存活的造血细胞主要是巨噬细胞。这些发现表明，S17，而不是S10可以支持骨髓和B淋巴细胞生成，并表明一个基质细胞群有能力形成一个造血微环境的两个谱系。
The production of B lymphocytes and myeloid cells occurs in the bone marrow in association with a supporting population of stromal cells. To determine whether these processes are dependent upon the same or different populations of stromal cells, stromal cell lines were generated from the adherent layer of a Dexter type long-term bone marrow culture. These cultures support myeloid cells and their precursors, a B cell precursor, and the adherent layer cells with support B cell differentiation under appropriate conditions. Two of the lines examined, S10 and S17, express class I histocompatibility antigens but not other hemopoietic cell surface determinants such as Thy-1, Lyt-1, Ig, Ia, Mac-1, or BP-1. Both lines could support myelopoiesis under Dexter conditions upon seeding with nylon wool-passed bone marrow. The nylon wool passage depletes stromal cells capable of forming adherent layers in vitro but retains hemopoietic precursors. The number of cells and colony-forming units-granulocytes/macrophages in the nonadherent cell population recovered 3 wk post-seeding had increased 19-fold and 10-fold, respectively, in the reseeded cultures of S10 and S17. After 3 wk of growth in Dexter conditions, the reseeded cultures were transferred to conditions optimal for B cell differentiation described by Whitlock and Witte. After 4 wk of growth, hemopoietic cells were consistently recovered from S17 cultures but not those of S10. A proportion of these cells from S17 cultures expressed the 14.8 antigen and were surface IgM positive. Surviving hemopoietic cells present in cultures of S10 were primarily macrophages. These findings indicate that S17 but not S10 can support both myelopoiesis and B lymphopoiesis and suggest that one stromal cell population has the capacity to form a hemopoietic microenvironment for both lineages.