Inducibility of the Drosophila melanogaster cytochrome P450 gene, CYP6A2, by phenobarbital in insecticide susceptible or resistant strains
Inducibility of the Drosophila melanogaster cytochrome P450 gene, CYP6A2, by phenobarbital in insecticide susceptible or resistant strains
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DOI：
10.1016/s0965-1748(96)00036-7
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发表时间：
1996-07-01
影响因子：
3.8
通讯作者：
Amichot, M
中科院分区：
文献类型：
--
作者：
Brun, A;Cuany, A;Amichot, M
细胞色素P450在细胞或生物体生物学中的重要性已经清楚地确立，虽然许多研究涉及脊椎动物，但对无脊椎动物细胞色素P450知之甚少。本文主要研究CYP6A2基因在果蝇中的表达。我们表明，该细胞色素P450基因在Canton（S）品系（野生型）中的表达受苯巴比妥的控制。在用苯巴比妥处理的成体中，该基因在中肠、表皮周脂肪体和马氏管中转录。诱导因子为15。在对杀虫剂DDT具有抗性的黑腹果蝇的RDDT（R）品系中，该基因在相同的组织中组成性过表达（相对于未处理的Canton（S）果蝇，过表达因子为6）。苯巴比妥对RDDT（R）的有效性不如对野生型品系的有效性（相对于未处理的RDDT（R）果蝇，诱导因子为2.5）。版权所有（C）1996 Elsevier Science Ltd.
The importance of cytochrome P450s in the biology of cells or organisms is clearly established, While numerous studies concern vertebrates, little is known about invertebrates cytochrome P450s. In this paper, we have focused on CYP6A2 gene expression in Drosophila melanogaster. We show the expression of this cytochrome P450 gene in the Canton(S) strain (wild type) to be under the control of phenobarbital, In adults treated with phenobarbital, this gene is transcribed in the midgut, the pericuticular fat bodies and the Malpighian tubules. The induction factor is 15. In the RDDT(R) strain of Drosophila melanogaster, which is resistant to the insecticide DDT, this gene is constitutively overexpressed in the same tissues (overexpression factor is 6 relative to untreated Canton(S) flies), Phenobarbital is not as effective on RDDT(R) (induction factor is 2.5 relative to untreated RDDT(R) dies) as on wild type strains. Copyright (C) 1996 Elsevier Science Ltd.