Baculovirus Production and Infection in Axolotls.
Baculovirus Production and Infection in Axolotls.
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DOI：
10.1007/978-1-0716-2659-7_24
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发表时间：
2023-01-01
期刊：
影响因子：
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通讯作者：
Tanaka, Elly M
中科院分区：
文献类型：
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作者：
Murawala, Prayag;Oliveira, Catarina R;Tanaka, Elly M
蝾螈是发育和组织再生研究的极好模型。虽然转基因方法可用于各种蝾螈物种，但它们的长世代时间和昂贵的维护推动了替代基因递送方法用于功能研究的发展。我们之前已经开发了假型杆状病毒（BV）作为在美西螈中进行基因递送的工具（Oliveira et al. Dev Biol 433（2）：262-275，2018）。自其最初的概念，我们已经完善了我们的BV生产和蝾螈模型的使用协议。在这一章中，我们描述了一个详细的和通用的协议BV介导的转导有尾目动物。
Salamanders have served as an excellent model for developmental and tissue regeneration studies. While transgenic approaches are available for various salamander species, their long generation time and expensive maintenance have driven the development of alternative gene delivery methods for functional studies. We have previously developed pseudotyped baculovirus (BV) as a tool for gene delivery in the axolotl (Oliveira et al. Dev Biol 433(2):262-275, 2018). Since its initial conception, we have refined our protocol of BV production and usage in salamander models. In this chapter, we describe a detailed and versatile protocol for BV-mediated transduction in urodeles.