Pombe Cdc5-related protein - A putative human transcription factor implicated in mitogen-activated signaling
Pombe Cdc5-related protein - A putative human transcription factor implicated in mitogen-activated signaling
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DOI：
10.1074/jbc.272.9.5833
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发表时间：
1997-02-28
影响因子：
4.8
通讯作者：
Coughlin, SR
中科院分区：
文献类型：
--
作者：
Bernstein, HS;Coughlin, SR
粟酒裂殖酵母cdc 5基因产物是一种细胞周期调节剂，在裂殖酵母的G（2）/M转换中发挥其作用。我们克隆了一个可能的人类转录因子，粟酒裂殖酵母Cdc 5相关蛋白（PCDC 5 RP），它与S. pombe Cdc 5和在小鼠、线虫和芽殖酵母中表达的序列。PCDC 5 RP在正常成人组织中广泛表达，因此可能具有进化上保留的重要一般功能。PCDC 5 RP含有两个螺旋-转角-螺旋DNA结合基序的串联重复序列、四个共有的核定位信号和一个亲水的富含脯氨酸的中心区域，该区域类似于Myb家族成员的转录激活结构域。值得注意的是，在培养细胞的血清刺激下，PCDC 5 RP从细胞质迅速移动到细胞核，这种移动与PCDC 5 RP磷酸化的增加在时间上相关，因此，PCDC 5 RP是一种假定的转录因子，在血清刺激时似乎将细胞质信号传递到细胞核。
The Schizosaccharomyces pombe cdc5 gene product is a cell cycle regulator that exerts its effects at the G(2)/M transition in fission yeast. We describe the cloning of a putative human transcription factor, pombe Cdc5-related protein (PCDC5RP), which bears significant homology to S. pombe Cdc5 and to expressed sequences in mouse, nematode, and budding yeast. PCDC5RP is expressed widely in normal adult human tissues and thus may have an important general function that has been preserved evolutionarily. PCDC5RP contains two tandem repeats of a helix-turn-helix DNA binding motif, four consensus nuclear localization signals, and a hydrophilic, proline-rich central region similar to the transcriptional activating domain in Myb family members, Remarkably, PCDC5RP moved rapidly from cytoplasm to nucleus upon serum stimulation of cultured cells, This movement correlated temporally with an increase in PCDC5RP phosphorylation, Thus, PCDC5RP is a presumed transcription factor that appears to transduce cytoplasmic signals to the nucleus upon serum stimulation.