Evidence that the respiratory syncytial virus polymerase complex associates with lipid rafts in virus-infected cells: a proteomic analysis
Evidence that the respiratory syncytial virus polymerase complex associates with lipid rafts in virus-infected cells: a proteomic analysis
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DOI：
10.1016/j.virol.2004.09.034
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发表时间：
2004-12-05
期刊：
影响因子：
3.7
通讯作者：
Sugrue, RJ
中科院分区：
文献类型：
--
作者：
McDonald, TP;Pitt, AR;Sugrue, RJ
在HEp2细胞中研究了呼吸道合胞病毒（RSV）聚合酶复合物与脂筏的相互作用。用病毒感染细胞制备脂筏膜，并用Western blotting和质谱法分析其蛋白质含量。这一分析揭示了N、P、L、M2-1和M蛋白的存在。然而，这些蛋白与这些结构的关联似乎彼此不同，与N、P或M蛋白相比，M2-1蛋白显示出更大的筏膜分裂。对梯度组分的聚合酶活性谱的测定表明，95%的可检测病毒酶活性与脂筏膜有关。此外，通过共聚焦显微镜对病毒感染细胞的分析表明，这些蛋白与筏脂GM1之间存在关联。总之，这些结果提供了证据，证明RSV聚合酶复合物能够与病毒感染细胞中的脂筏相关联。(C) 2004爱思唯尔公司版权所有。
The interaction between the respiratory syncytial virus (RSV) polymerase complex and lipid rafts was examined in HEp2 cells. Lipid-raft membranes were prepared from virus-infected cells and their protein content was analysed by Western blotting and mass spectrometry. This analysis revealed the presence of the N, P, L, M2-1 and M proteins. However, these proteins appeared to differ from one another in their association with these structures, with the M2-1 protein showing a greater partitioning into raft membranes compared to that of the N, P or M proteins. Determination of the polymerase activity profile of the gradient fractions revealed that 95% of the detectable viral enzyme activity was associated with lipid-raft membranes. Furthermore, analysis of virus-infected cells by confocal microscopy suggested an association between these proteins and the raft-lipid, GM1. Together, these results provide evidence that the RSV polymerase complex is able to associate with lipid rafts in virus-infected cells. (C) 2004 Elsevier Inc. All rights reserved.