INTEGRATION OF HEPATITIS-B VIRUS-DNA INTO THE GENOME OF LIVER-CELLS IN CHRONIC LIVER-DISEASE AND HEPATOCELLULAR-CARCINOMA - STUDIES IN PERCUTANEOUS LIVER BIOPSIES AND POSTMORTEM TISSUE SPECIMENS
INTEGRATION OF HEPATITIS-B VIRUS-DNA INTO THE GENOME OF LIVER-CELLS IN CHRONIC LIVER-DISEASE AND HEPATOCELLULAR-CARCINOMA - STUDIES IN PERCUTANEOUS LIVER BIOPSIES AND POSTMORTEM TISSUE SPECIMENS
复制标题
DOI：
10.1056/nejm198110293051807
复制
发表时间：
1981-01-01
影响因子：
158.5
通讯作者：
KEW, MC
中科院分区：
文献类型：
--
作者：
SHAFRITZ, DA;SHOUVAL, D;KEW, MC
应用重组DNA技术和凝胶电泳法，在肝细胞癌和慢性肝病患者的肝脏和肿瘤组织中检测到HBVDNA，并研究了HBVDNA整合到这些细胞基因组中的情况。12例血清乙肝表面抗原(HBs)阳性的肝细胞癌患者的肿瘤组织中检测到整合型HBVDNA，8例血清HBs Ag阴性但抗-HBs抗体阳性的患者肿瘤中也检测到了整合的HBVDNA。在某些病例中，整合的HBVDNA也存在于与肿瘤具有相同杂交模式或不同杂交模式的非肿瘤肝组织中。在5名有携带者状态和慢性肝病证据2年的乙肝表面抗原携带者中，肝组织中存在HBVDNA，但未整合。在2名携带乙肝表面抗原8年的患者中，HBVDNA整合到宿主基因组中。在肉眼肿瘤的发生发展过程中，肝细胞中可能存在着HBVDNA的整合。
Recombinant-DNA technology and gel electrophoresis were used to find hepatitis B virus DNA (HBV-DNA) in liver and tumor tissue from patients with hepatocellular carcinoma and chronic liver disease and to study the integration of HBV-DNA into the genome of these cells. In 12 patients with hepatocellular carcinoma who had hepatitis B surface antigen (HBsAg) in their serum, integrated HBV-DNA was identified in the tumors; it was also found in tumors from 3 of 8 patients who were seronegative for HBsAg but positive for antibody to HBsAg. In some cases, integrated HBV-DNA was also present in nontumorous liver tissue that had the same hybridization pattern or one different from that of the tumor. In 5 carriers of HBsAg who had evidence of the carrier state and chronic liver disease for < 2 yr, HBV-DNA was present but not integrated in liver tissue. In the 2 patients who had carried HbsAg for > 8 yr, HBV-DNA was integrated into the host genome. Integration of HBV-DNA into hepatocytes may occur during development of gross neoplasm.