Common computational tools for analyzing CRISPR screens.
Common computational tools for analyzing CRISPR screens.
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DOI：
10.1042/etls20210222
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发表时间：
2021-12-21
影响因子：
3.8
通讯作者：
Hart T
中科院分区：
文献类型：
--
作者：
Colic M;Hart T
CRISPR-Cas技术为基因组编辑提供了一个多功能的工具箱，可应用于各种癌症相关领域，如功能基因组学，免疫治疗，合成致死性和耐药性，转移，基因组调控，染色质可及性和RNA靶向。筛选平台的多样性和使用它们的问题已经导致了用于CRISPR分析的各种分析方法的发展。在这篇综述中，我们重点介绍了在合并CRISPR敲除（KO）筛选的计算分析中使用的算法和框架，并强调了使用这些方法发现的一些最重要的靶点。最后，我们提供的设计和分析的最先进的多重筛选遗传相互作用的观点。
CRISPR–Cas technology offers a versatile toolbox for genome editing, with applications in various cancer-related fields such as functional genomics, immunotherapy, synthetic lethality and drug resistance, metastasis, genome regulation, chromatic accessibility and RNA-targeting. The variety of screening platforms and questions in which they are used have caused the development of a wide array of analytical methods for CRISPR analysis. In this review, we focus on the algorithms and frameworks used in the computational analysis of pooled CRISPR knockout (KO) screens and highlight some of the most significant target discoveries made using these methods. Lastly, we offer perspectives on the design and analysis of state-of-art multiplex screening for genetic interactions.
影响因子：
4.4
作者：
Trümbach D;Pfeiffer S;Poppe M;Scherb H;Doll S;Wurst W;Schick JA
通讯作者：
Schick JA