Nonoxynol-9 causes rapid exfoliation of sheets of rectal epithelium
Nonoxynol-9 causes rapid exfoliation of sheets of rectal epithelium
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DOI：
10.1016/s0010-7824(00)00156-6
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发表时间：
2000-09-01
期刊：
影响因子：
2.9
通讯作者：
Maguire, RA
中科院分区：
文献类型：
--
作者：
Phillips, DM;Taylor, CL;Maguire, RA
含壬氧醇-9 （N-9）的杀精剂和其他含N-9的产品通常用作直肠性交中的润滑剂。我们之前已经证明，在小鼠直肠应用N-9产品会导致上皮细胞脱落，增加HSV-2感染的可能性。为了确定含有N-9的产品是否会对人类直肠上皮产生类似的影响，将K-Y(R)Plus和ForPlay(R)这两种非处方（OTC） N-9产品的应用与不含N-9的卡拉胶和甲基纤维素两种配方的应用进行了比较。在4名人类参与者中评估了每种配方的效果。应用N-9产品15分钟后收集的直肠灌洗标本的光镜和电镜检查显示存在上皮片。每张薄片含有数百个上皮细胞，包括柱状细胞和杯状细胞，这些细胞是直肠上皮细胞的典型形态。在使用N-9产品后8至12小时收集的直肠灌洗标本中，或在任何涉及不含N-9配方的定时灌洗中，均未观察到上皮片。此外，在基线灌洗标本中未观察到上皮细胞片。我们得出的结论是，直肠使用含有n -9的产品会导致直肠上皮广泛区域的快速脱落。8小时后不再观察到上皮脱落。我们有理由认为，失去保护上皮会使人更容易感染艾滋病毒和其他性传播病原体。因此，我们告诫不要在直肠性交时使用含有n -9的产品。(C) 2000 Elsevier Science Inc.；版权所有。
Nonoxynol-9 (N-9) containing spermicides and other N-9 containing products are commonly used as lubricants during rectal intercourse. We have previously demonstrated that rectal application of N-9 products in mice can cause exfoliation of epithelial cells, increasing the probability of infection by HSV-2. To determine if N-9-containing products would have a similar effect on the rectal epithelium in humans, the application of K-Y(R)Plus and ForPlay(R), both over-the-counter (OTC) N-9 products, were compared to the application of two formulations, carrageenan and methyl cellulose, that do not contain N-9. The effects of each formulation were evaluated in 4 human participants. Light and electron microscope examination of rectal lavage specimens collected 15 min post application of N-9 products revealed the presence of sheets of epithelium. Each sheet contained hundreds of epithelial cells that included columnar and goblet cells, varieties of cells typical of rectal epithelial morphology. Sheets of epithelium were not observed in rectal lavage specimens collected 8 to 12 hr post N-9 product use or in either of the timed lavages involving non-N-9 containing formulations. In addition, no sheets of epithelial cells were observed in the baseline lavage specimens. We conclude that the rectal use of N-9-containing products causes a rapid exfoliation of extensive areas of the rectal epithelium. Exfoliation of the epithelium is no longer observed at 8 hr. It is reasonable to assume that the loss of the protective epithelium would render a person more at risk for infection by HIV and other sexually transmitted pathogens. We, therefore, caution against the use of N-9-containing products during rectal intercourse. (C) 2000 Elsevier Science Inc. All rights reserved.