Squalene cyclase and oxidosqualene cyclase from a fern
Squalene cyclase and oxidosqualene cyclase from a fern
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DOI：
10.1016/j.febslet.2007.12.023
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发表时间：
2008-01-01
期刊：
影响因子：
3.5
通讯作者：
Ebizuka, Yutaka
中科院分区：
文献类型：
--
作者：
Shinozaki, Junichi;Shibuya, Masaaki;Ebizuka, Yutaka
蕨类植物是最原始的维管束植物。蕨类植物的植物甾醇与高等植物的植物甾醇相同，但它们产生特有的三萜。最明显的特征是C-3处缺乏氧官能团，这表明蕨类植物中的三萜可能是通过角鲨烯的直接环化而生物合成的。为了深入了解蕨类植物中三萜生物合成的分子基础，我们克隆了ACX和ACH，ACX是一个氧化齐墩果烯环化酶同源物，编码一个环artenol合成酶(CAS)，而ACH是一个角鲨烯环化酶同源物，编码一个来自铁线铁线藻属的22-羟基Hopane合成酶。系统发育分析表明，ACH位于细菌SCs簇中，而ACX位于高等植物CASS簇中。(C)2007年欧洲生化学会联合会。爱思唯尔出版，版权所有。
Ferns are the most primitive vascular plants. The phytosterols of ferns are the same as those of higher plants, but they produce characteristic triterpenes. The most distinct feature is the lack of oxygen functionality at C-3, suggesting that the triterpenes of ferns may be biosynthesized by direct cyclization of squalene. To obtain some insights into the molecular bases for the biosynthesis of triterpenes in ferns, we cloned ACX, an oxidosqualene cyclase homologue, encoding a cycloartenol synthase (CAS) and ACH, a squalene cyclase homologue, encoding a 22-hydroxyhopane synthase from Adiantum capillus-veneris. Phylogenetic analysis revealed that ACH is located in the cluster of bacterial SCs, while ACX is in the cluster of higher plant CASs. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.