Relative phosphocreatine and nucleoside triphosphate concentrations in cerebral gray and white matter measured in vivo by 31P nuclear magnetic resonance.
Relative phosphocreatine and nucleoside triphosphate concentrations in cerebral gray and white matter measured in vivo by 31P nuclear magnetic resonance.
复制标题
通过 31P 核磁共振在体内测量大脑灰质和白质中的相对磷酸肌酸和三磷酸核苷浓度。
DOI：
10.1016/0006-8993(95)01229-x
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发表时间：
1996
期刊：
影响因子：
2.9
通讯作者：
Holtzman,D
中科院分区：
文献类型：
--
作者：
Tsuji,MK;Mulkern,RV;Cook,CU;Meyers,RL;Holtzman,D
与白质相比，大脑灰质的 ATP 代谢率通常较高。为了研究体内这种区域差异的生理学，采用一维化学位移成像技术（1D-CSI）从4周龄仔猪大脑的2.5毫米切片中获取31P核磁共振谱。通过磁共振图像评估，将主要灰质切片（估计 76% 灰质，头皮下方 7 毫米）的光谱与主要白质切片（56% 估计白质，头皮下方 13 毫米）进行比较。 1D-CSI 技术并未对含有磷酸肌酸 (PCr) 和 ATP 溶液的单室体模的信号比率产生系统性变化。使用 2 s 脉冲间隔，灰质切片显示 PCr/NTP 比率为 0.93 ± 0.11（平均值 ± S.D.），该值非常接近表面线圈局域光谱中的比率。以白质为主的切片显示 PCr/NTP 比率为 1.32 ± 0.18（灰质与白质的 P < 0.02）。使用两个切片中灰质和白质的估计百分比以及根据完全弛豫光谱计算的浓度，灰质 PCr/NTP 比率约为 0.77，而白质中的比率约为 2.18。体内测量的 PCr/NTP 差异表明，仔猪大脑灰质中的总 NTP 浓度高于白质，或者稳态 PCr 浓度低于白质。
Rates of ATP metabolism generally are higher in cerebral gray matter compared to white matter. In order to study the physiology of this regional difference in vivo, the 1-dimensional chemical shift imaging technique (1D-CSI) was used to acquire31P nuclear magnetic resonance spectra from 2.5 mm slices of 4-week old piglet brains. Spectra from predominantly gray matter slices (estimated 76% gray matter, 7 mm below the scalp) were compared to predominantly white matter slices (56% estimated white matter, 13 mm below the scalp) as assessed by magnetic resonance images. The 1D-CSI technique introduced no systematic changes in the ratio of signals from a single chamber phantom containing a phosphocreatine (PCr) and ATP solution. Gray matter slices showed a PCr/NTP ratio of 0.93 ± 0.11 (mean ± S.D.) using a 2 s interpulse interval, a value very close to the ratio in surface coil localized spectra. The predominantly white matter slices showed a PCr/NTP ratio of 1.32 ± 0.18 (P < 0.02 for gray versus white matter). Using the estimated percentages of gray and white matter in the two slices and calculated concentrations from fully relaxed spectra, the gray matter PCr/NTP ratio is approximately 0.77, while the ratio in white matter is approximately 2.18. The difference in PCr/NTP measured in vivo suggests that either the total NTP concentration is higher or the steady state PCr concentration is lower in gray matter than in white matter in the piglet brain.