Neuropeptides in the cerebral ganglia of the mud crab, Scylla paramamosain: transcriptomic analysis and expression profiles during vitellogenesis.
Neuropeptides in the cerebral ganglia of the mud crab, Scylla paramamosain: transcriptomic analysis and expression profiles during vitellogenesis.
复制标题
青蟹大脑神经节中的神经肽，卵黄发生过程中的转录组分析和表达谱
DOI：
10.1038/srep17055
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发表时间：
2015-11-23
影响因子：
4.6
通讯作者：
Ye H
中科院分区：
文献类型：
--
作者：
Bao C;Yang Y;Huang H;Ye H
神经肽在调节动物繁殖中起着重要作用。在脊椎动物中，GnRH、GnIH和kisspeptin是生殖轴的关键神经肽激素，然而，无脊椎动物的生殖轴是模糊的。拟穴青蟹（Scylla paramamosain）卵巢发育的研究对于该物种的水产养殖和资源管理具有重要意义。本研究采用Illumina测序、逆转录-聚合酶链反应和实时定量PCR技术鉴定可能参与卵巢发育的神经肽。拟穴龙从雌性的转录组数据中预测了来自24个神经肽家族的32种神经肽转录物，100种不同的成熟肽。大脑旁隙神经节其中，GSEFLamide和WXXXRamide两个家族是首次从甲壳类动物脑神经节中发现的。在这些神经肽中，选择了21种感兴趣的转录物进行进一步确认，并且所有这些转录物都在大脑神经节以及其他神经组织和卵巢中检测到。其中大多数也有不同的表达在大脑神经节在不同的卵黄发生阶段，这表明他们可能参与调节卵黄发生和卵巢成熟。总之，这些发现为后续研究肽在S.拟穴龙
Neuropeptides play a critical role in regulating animal reproduction. In vertebrates, GnRH, GnIH and kisspeptin are the key neuropeptide hormones of the reproductive axis, however, the reproductive axis for invertebrates is vague. Knowledge on ovarian development of the mud crab,Scylla paramamosain, is critical for aquaculture and resources management of the commercially important species. This study employed Illumina sequencing, reverse transcription-polymerase chain reaction and quantitative real-time PCR techniques to identify neuropeptides that may be involved in ovarian development ofS. paramamosain. A total of 32 neuropeptide transcripts from two dozen neuropeptide families, 100 distinct mature peptides were predicted from the transcriptome data of femaleS. paramamosaincerebral ganglia. Among them, two families,i.e.GSEFLamide and WXXXRamide, were first identified from the cerebral ganglia of crustaceans. Of these neuropeptides, 21 transcripts of interest were selected for further confirmation and all of them were detected in the cerebral ganglia, as well as in other nervous tissues and the ovary. Most of them also had differential expression in the cerebral ganglia during various vitellogenic stages, suggesting their likely involvement in regulating vitellogenesis and ovarian maturation. Overall, these findings provide an important basis for subsequent studies on peptide function in reproduction ofS. paramamosain.