Porcine peripheral blood dendritic cells and natural interferon-producing cells
Porcine peripheral blood dendritic cells and natural interferon-producing cells
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DOI：
10.1111/j.1365-2567.2003.01755.x
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发表时间：
2003-12-01
期刊：
影响因子：
6.4
通讯作者：
McCullough, KC
中科院分区：
文献类型：
--
作者：
Summerfield, A;Guzylack-Piriou, L;McCullough, KC
外周血含有两个主要的特定罕见的树突状细胞（DC）亚群，其连接先天性和特异性免疫系统，相当于天然干扰素产生细胞（NIPC）的髓样DC和浆细胞样DC。这些细胞的功能表征需要大量的血液，使得大型动物模型更适合和有益于某些研究。在这里，描述了表达猪车间簇3（SWC 3，SIRP家族成员）的猪血液单核细胞的两个子集，并与单核细胞进行比较。血DC的主要组织相容性复合物（MHC）Ⅱ类阳性，CD 80/86（+），CD 1（+/-），CD 4（-），而单核细胞CD 14（-）。可区分CD 16（-）和CD 16（+）亚群。粒细胞-巨噬细胞集落刺激因子和白细胞介素-3是该DC亚群的存活因子，培养诱导MHC II类和CD 80/86的上调。所述的第二个亚组是猪NIPC，通常为CD 4（++）、MHC II类低、CD 80/86（低）、CD 1（-）、CD 8（-/低）、CD 16（-/低）和CD 45 RA（-/低）。猪NIPC具有高的白细胞介素-3结合能力，并在对这种细胞因子的反应中存活。它们的独特功能是在病毒刺激后分泌强的I型干扰素。这两个子集是内吞活性新鲜分离时，在体外成熟后下调这种活动。两者合计，本报告已经划定了猪血液DC和NIPC，允许更详细地了解先天免疫防御，特别是对感染的反应。
Peripheral blood contains two major particular infrequent dendritic cells (DC) subsets linking the innate and specific immune system, the myeloid DC and plasmacytoid DC equivalent to the natural interferon-producing cells (NIPC). The functional characterization of these cells demands large volumes of blood, making a large animal model more appropriate and beneficial for certain studies. Here, two subsets of porcine blood mononuclear cells expressing swine workshop cluster 3 (SWC3, a SIRP family member), are described and compared to monocytes. The blood DC specialized in T-cell stimulation were major histocompatibility complex (MHC) class II+, CD80/86(+), CD1(+/-), CD4(-), and in contrast to monocytes CD14(-). A CD16(-) and a CD16(+) subset could be discriminated. Granulocyte-macrophage colony-stimulating factor and interleukin-3 were survival factors for this DC subset, and culture induced an up-regulation of MHC class II and CD80/86. The second subset described, are porcine NIPC, typically CD4(++), MHC class IIlow, CD80/86(low), CD1(-), CD8(-/low), CD16(-/low) and CD45RA(-/low). Porcine NIPC had high interleukin-3 binding capacity, and survived in response to this cytokine. Their unique function was strong interferon type I secretion after virus stimulation. Both subsets were endocytically active when freshly isolated, and down-regulated this activity after in vitro maturation. Taken together, the present report has delineated porcine blood DC and NIPC, permitting a more detailed understanding of innate immune defences, particularly in response to infections.