Distinctive, rapid, and easy labeling of diffuse plaques in the Alzheimer brains by a new methenamine silver stain
Distinctive, rapid, and easy labeling of diffuse plaques in the Alzheimer brains by a new methenamine silver stain
复制标题
通过新的乌洛托品银染色剂对阿尔茨海默病大脑中的弥漫性斑块进行独特、快速、轻松的标记
作者：
Haruyasu Yamaguchi;C. Haga;Shunsaku Hirai;Y. Nakazato;Kenji Kosaka
我们开发了一种新的甲基胺银（MS）染色方法，用于检测阿尔茨海默氏型痴呆、唐氏综合征和智力正常的老年大脑石蜡包埋组织切片上的弥漫性斑块。这种快速简便的方法选择性地标记老年斑的淀粉样蛋白相关成分，但不标记Gerstmann-Sträussler综合征中发现的库鲁斑块。我们的MS染色显示与甲酸预处理的β蛋白免疫染色几乎相同的染色模式。因此，新的MS染色适用于老年斑包括弥漫性斑块的常规或筛选研究。
SummaryWe have developed a new methenamine silver (MS) stain for detecting diffuse plaques distinctively on paraffin-embedded tissue sections of Alzheimer-type dementia, Down'n syndrome, and mentally normal aged brains. This rapid and easy method selectively labels amyloid-related component of senile plaques, but not of kuru plaques found in Gerstmann-Sträussler syndrome. Our MS stain shows almost the same staining pattern as that of the β protein immunostaining with formic acid pretreatment. Therefore, new MS stain is appropriate to routine or screening studies for senile plaques including diffuse plaques.