Differential immune responses of the green neon shrimp (Neocaridina denticulate) to dipropyl phthalate.
Differential immune responses of the green neon shrimp (Neocaridina denticulate) to dipropyl phthalate.
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DOI：
10.1016/j.fsi.2011.06.021
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发表时间：
2011-09
影响因子：
4.7
通讯作者：
H. Sung;Yi-Hsiu Lin;C. Hsiao
中科院分区：
文献类型：
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作者：
H. Sung;Yi-Hsiu Lin;C. Hsiao
本研究旨在了解外源性邻苯二甲酸酯（PAEs）的亚致死效应与对虾对细菌感染的易感性和免疫应答之间的关系。将齿状新米虾暴露于不同浓度的邻苯二甲酸二丙酯（DPrP），分别于暴露后第1、3、5和10天测定死亡率和6项免疫指标。在暴露后第1天和第3天，暴露于0、1、5、10和50 mg/L DPrP和用Veronii气单胞菌攻击的对虾分别经历14%和16%、16%和16%、18%和18%、34%和24%、38%和26%的死亡率。第1天，与未处理的虾相比，所有DPrP处理组的五项免疫参数（酸性磷酸酶，AcP; β-葡萄糖醛酸酶，β-Glu;酚氧化酶，PO;超氧化物歧化酶，SOD;和血蓝蛋白mRNA）均发生显着改变，并且在10 mg/L和50 mg/L处理组中升高。β-Glu活性和血蓝蛋白mRNA水平以剂量依赖性方式显著增加。这些结果表明，N. DPrP对小鼠齿状回的影响是短期的，可能与DPrP诱导的免疫介质表达增加有关。
This study set out to understand the sublethal effect of xenobiotic phthalate esters (PAEs) on the relationship between the susceptibility of shrimp to bacterial infection and the immune response of the shrimp. Neocaridina denticulate were exposed to different concentrations of the PAE dipropyl phthalate (DPrP), and mortality and six immune parameters were measured on days 1, 3, 5, and 10 after exposure. On days 1 and 3 after exposure, shrimp exposed to 0, 1, 5, 10, and 50 mg/L of DPrP and challenged with Aeromonas veronii experienced 14% and 16%, 16% and 16%, 18% and 18%, 34% and 24%, and 38% and 26% mortality, respectively. On day 1, five immune parameters (acid phosphatase, AcP; β-glucuronidase, β-Glu; phenoloxidase, PO; superoxide dismutase, SOD; and haemocyanin mRNA) were significantly altered in the all of the groups treated with DPrP compared to the untreated shrimp and were elevated in the 10 mg/L- and 50 mg/L-treated groups. Beta–Glu activity and haemocyanin mRNA levels were significantly increased in a dose-dependent manner. These results suggest that the increased susceptibility of N. denticulate exposed to DPrP is short-term and may be related to the increased expression of DPrP-induced immune mediators.