Fluorescence ratio thermometry in a microfluidic dual-beam laser trap
Fluorescence ratio thermometry in a microfluidic dual-beam laser trap
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DOI：
10.1364/oe.15.015493
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发表时间：
2007-11-12
期刊：
影响因子：
3.8
通讯作者：
Guck, Jochen
中科院分区：
文献类型：
--
作者：
Ebert, Susanne;Travis, Kort;Guck, Jochen
双光束激光陷阱是一种多功能工具，具有许多可能的应用。为了在微流控阱几何结构中表征其热性能，我们开发了一种非侵入性荧光比技术，使用温度敏感染料罗丹明B和温度无关参考染料罗丹明110。我们在共聚焦激光扫描显微镜上以亚微米的空间分辨率测量了陷阱中的温度分布。当波长λ = 1064 nm时，阱中心的最大加热达到（13 +/- 2）℃/W，并与施加的功率成线性比例。测量结果与模拟温度分布吻合得很好。(c) 2007年美国光学学会。
The dual-beam laser trap is a versatile tool with many possible applications. In order to characterize its thermal properties in a microfluidic trap geometry we have developed a non-intrusive fluorescence ratio technique using the temperature sensitive dye Rhodamine B and the temperature independent reference dye Rhodamine 110. We measured temperature distribution profiles in the trap with submicron spatial resolution on a confocal laser-scanning microscope. The maximum heating in the center of the trap amounts to (13 +/- 2) degrees C/W for a wavelength of lambda = 1064 nm and scales linearly with the applied power. The measurements correspond well with simulated temperature distributions. (c) 2007 Optical Society of America.