Improvement of Aptamer Affinity by Dimerization.
Improvement of Aptamer Affinity by Dimerization.
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DOI：
10.3390/s8021090
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发表时间：
2008-02-19
期刊：
影响因子：
--
通讯作者：
Ikebukuro K
中科院分区：
文献类型：
--
作者：
Hasegawa H;Taira KI;Sode K;Ikebukuro K
为了增加适体对其靶标的亲和力，我们设计了凝血酶和VEGF的适体二聚体。这种设计基于抗体的亲合力，这使得适体能够轻松连接，因为它是单链核酸。在这项研究中，我们连接了一个15-mer的凝血酶结合适体与一个29-mer的凝血酶结合适体。每个适体识别凝血酶分子的不同部分，并且适体二聚体的Kd值是组成它的单体的Kd值的1/10。此外，所设计的适体二聚体具有比所报道的（15-mer）凝血酶抑制适体更高的抑制活性。此外，我们将两个相同的针对血管内皮生长因子（VEGF 165）的适体连接在一起，VEGF 165是一种同源二聚体蛋白。如在抗凝血酶适体的情况下，二聚体抗VEGF适体具有比单体低得多的Kd值。该研究表明，适体的二聚化有效地提高了这些适体对其靶标的亲和力。
To increase the affinities of aptamers for their targets, we designed an aptamer dimer for thrombin and VEGF. This design is based on the avidity of the antibody, which enables the aptamer to connect easily since it is a single-strand nucleic acid. In this study, we connected a 15-mer thrombin-binding aptamer with a 29-mer thrombin-binding aptamer. Each aptamer recognizes a different part of the thrombin molecule, and the aptamer dimer has a Kd value which is 1/10 of that of the monomers from which it is composed. Also, the designed aptamer dimer has higher inhibitory activity than the reported (15-mer) thrombin-inhibiting aptamer. Additionally, we connected together two identical aptamers against vascular endothelial growth factor (VEGF165), which is a homodimeric protein. As in the case of the anti-thrombin aptamer, the dimeric anti-VEGF aptamer had a much lower Kd value than that of the monomer. This study demonstrated that the dimerization of aptamers effectively improves the affinities of those aptamers for their targets.
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影响因子：
5.6
作者：
Tasset, DM;Kubik, MF;Steiner, W
通讯作者：
Steiner, W
DOI：
10.1073/pnas.94.14.7192
发表时间：
1997-07-08
影响因子：
11.1
作者：
Muller, YA;Li, B;DeVos, AM
通讯作者：
DeVos, AM
影响因子：
46.9
作者：
Fredriksson, S;Gullberg, M;Landegren, U
通讯作者：
Landegren, U
影响因子：
2.7
作者：
Stauffer, ME;Skelton, NJ;Fairbrother, WJ
通讯作者：
Fairbrother, WJ
影响因子：
7.4
作者：
Yoshida, Wataru;Sode, Koji;Ikebukuro, Kazunori
通讯作者：
Ikebukuro, Kazunori