The Arabidopsis thaliana ABC protein superfamily, a complete inventory
The Arabidopsis thaliana ABC protein superfamily, a complete inventory
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DOI：
10.1074/jbc.m103104200
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发表时间：
2001-08-10
影响因子：
4.8
通讯作者：
Rea, PA
中科院分区：
文献类型：
--
作者：
Sánchez-Fernández, R;Davies, TGE;Rea, PA
我们描述了来自多细胞生物（模式植物拟南芥）的ATP结合盒（ABC）蛋白的第一个完整清单。通过应用几种搜索标准，发现拟南芥中总共含有129个能够编码ABC蛋白的开放阅读框（ORF），其中103个具有连续的跨膜跨度，并被鉴定为推定的内在膜蛋白。52个推定的内在膜蛋白含有至少两个跨膜结构域（TMD）和两个核苷酸结合折叠（NBFs），并可以归类为属于全分子转运蛋白的五个亚家族之一。其他51个推定的膜蛋白，所有这些都是半分子转运蛋白，落入五个亚科。其余的ORF鉴定，所有这些编码的蛋白质缺乏TMDs，11个可以分为三个亚科。有没有明显的同源物在其他生物的15个ORF编码的非固有的ABC蛋白（NA-Ps）的异质组。无根系统发育分析证实了亚科名称。拟南芥ABC超家族的显著特征是存在一个大的酵母Eke PDR亚家族，并且不存在编码真正的囊性纤维化跨膜传导调节因子（CFTR）、磺酰脲受体（SUR）和重金属耐受因子1（HMT 1）同源物的基因。拟南芥是不寻常的，在其大的开放阅读框（最低0.5%）的ABC蛋白超家族成员的分配。
We describe the first complete inventory of ATP-binding cassette (ABC) proteins from a multicellular organism, the model plant Arabidopsis thaliana. By the application of several search criteria, Arabidopsis was found to contain a total of 129 open reading frames (ORFs) capable of encoding ABC proteins, of which 103 possessed contiguous transmembrane spans and were identified as putative intrinsic membrane proteins. Fifty-two of the putative intrinsic membrane proteins contained at least two transmembrane domains (TMDs) and two nucleotide-binding folds (NBFs) and could be classified as belonging to one of five subfamilies of full-molecule transporters. The other 51 putative membrane proteins, all of which were half-molecule transporters, fell into five subfamilies. Of the remaining ORFs identified, all of which encoded proteins lacking TMDs, 11 could be classified into three subfamilies. There were no obvious homologs in other organisms for 15 of the ORFs which encoded a heterogeneous group of non-intrinsic ABC proteins (NA-Ps). Unrooted phylogenetic analyses substantiated the subfamily designations. Notable features of the Arabidopsis ABC superfamily was the presence of a large yeast-Eke PDR subfamily, and the absence of genes encoding bona fide cystic fibrosis transmembrane conductance regulator (CFTR), sulfonylurea receptor (SUR), and heavy metal tolerance factor 1 (HMT1) homologs. Arabidopsis was unusual in its large allocation of ORFs (a minimum of 0.5%) to members of the ABC protein superfamily.