Characterization and functional analysis of the promoter for the human equilibrative nucleoside transporter gene, hENT1
Characterization and functional analysis of the promoter for the human equilibrative nucleoside transporter gene, hENT1
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DOI：
10.1080/15257770601052331
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发表时间：
2007-01-01
影响因子：
1.3
通讯作者：
Coe, Imogen R.
中科院分区：
文献类型：
--
作者：
Abdulla, Parween;Coe, Imogen R.
平衡核苷转运蛋白（ENTs）是一种跨膜转运核苷、核苷碱基和类似物的膜蛋白。ENT基因及其表达调控知之甚少。因此，我们分离和功能特征的启动子的原型人类ENT，hENT1。TATA盒下游58 bp和翻译起始位点上游272 bp处存在单个转录起始位点。hENT1和小鼠ENT1（mENT1）启动子之间存在有限的序列相似性，表明哺乳动物中ENT1转录调节因子的保守性。转录因子的推定共有位点存在于hENT1启动子内。报告基因分析显示，在人类细胞中的转录活性相似，但不相同。
Equilibrative nucleoside transporters (ENTs) are membrane proteins that transport nucleosides, nucleobases and analogs across membranes. ENT genes and the regulation of their expression are poorly understood. Therefore, we isolated and functionally characterized the promoter of the prototypic human ENT, hENT1. A single transcriptional initiation site 58 bp downstream of the TATA box and 272 bp upstream of the translation initiation site is present. Limited sequence similarity exists between the hENT1 and mouse ENT1 (mENT1) promoters suggesting conservation of ENT1 transcriptional regulators in mammals. Putative consensus sites for transcription factors exist within the hENT1 promoter. Reporter assays revealed similar but not identical transcriptional activity profiles in human cells.