Macrophage-derived growth factor for fibroblasts and Interleukin-1 are distinct entities.
Macrophage-derived growth factor for fibroblasts and Interleukin-1 are distinct entities.
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成纤维细胞的巨噬细胞衍生生长因子和 Interleukin-1 是不同的实体。
DOI：
10.1002/jlb.35.1.115
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发表时间：
1984
影响因子：
5.5
通讯作者：
Gillespie,GY
中科院分区：
文献类型：
--
作者：
Estes,JE;Pledger,WJ;Gillespie,GY
P388D1 是一种小鼠巨噬细胞样细胞系，适合在未补充的无血清培养基中持续生长，并持续产生对静止小鼠成纤维细胞（BALB/c 3T3 细胞）和凝集素次优刺激的胸腺细胞有丝分裂作用的物质。我们之前已经将成纤维细胞有丝分裂活性描述为巨噬细胞衍生能力因子（MDCF）[37]。通过超滤（中空纤维）和冻干相结合，将无血清巨噬细胞条件培养基浓缩 1,000 倍。对培养基浓缩物进行凝胶过滤（Sephadex G-75 或 G-150），并测定组分对密度停滞的 BALB/c 3T3 细胞的有丝分裂活性 (MDCF) 以及次优刺激 (Con A) 小鼠胸腺细胞中的白介素-1 (IL-1) 活性。 MDCF 活性的表观分子量 (MW) 估计为 56,000 道尔顿，而 IL-1 色谱峰的表观分子量为 14-16K 道尔顿。 MDCF 级分中未检测到 IL-1 活性，IL-1 级分中也未检测到 MDCF。这些数据表明 P388D1 细胞在连续无血清条件下产生 MDCF 和 IL-1 活性，并且这两种活性并不相同。用脂多糖和/或富含淋巴因子的上清液刺激反应性单核吞噬细胞导致 MDCF 和 IL-1 活性的差异调节。最后，抗体纯化的 IL-1 在对胸腺细胞有丝分裂的浓度下没有显着刺激静止成纤维细胞 DNA 合成的能力。然而，IL-1 确实增强了次优量的血小板衍生生长因子 (PDGF)（另一种能力因子）的促有丝分裂活性。进一步的研究表明，MDCF 的产生和活性均不受各种蛋白水解酶抑制剂的存在调节。
P388D1, a mouse macrophagelike cell line, was adapted to grow continuously in an unsupplemented, serum‐free culture medium and continued to elaborate substances that were mitogenic for quiescent mouse fibroblasts (BALB/c 3T3 cells) and for thymocytes suboptimally stimulated with lectins. We have previously described [37] the fibroblast mitogenic activity as a macrophage‐derived competence factor (MDCF). Serum‐free, macrophage‐conditioned culture medium was concentrated 1,000‐fold by a combination of ultrafiltration (hollow fiber) and lyophilization. Concentrates of medium were subjected to gel filtration (Sephadex G‐75 or G‐150), and the fractions were assayed for mitogenic activity (MDCF) on density‐arrested BALB/c 3T3 cells and for Interleukin‐1 (IL‐1) activity in suboptimally stimulated (Con A) mouse thymocytes. The apparent molecular weight (MW) of MDCF activity was estimated at 56,000 daltons, whereas the peak of IL‐1 chromatographed at an apparent MW of 14–16K daltons. There was no detectable IL‐1 activity in the MDCF fractions and no detectable MDCF in the IL‐1 fractions. These data indicate that P388D1cells produce both MDCF and IL‐1 activities under continuous serum‐free conditions and that the two activities are not identical. Stimulation of responsive mononuclear phagocytes with lipopolysaccharide and/or lymphokine‐rich supernates resulted in a differential modulation of MDCF and IL‐1 activities. Finally, antibody‐purified IL‐1 had no significant ability to stimulate DNA synthesis in quiescent fibroblasts at concentrations that were mitogenic for thymocytes. However, IL‐1 did augment the mitogenic activity of suboptimal amounts of platelet‐derived growth factor (PDGF), another competence factor. Further studies revealed that neither the generation nor the activity of MDCF was modulated by the presence of various inhibitors of proteolytic enzymes.