Production of tetrapyrrole compounds and vitamin B12 using genetically engineering of Propionibacterium freudenreichii.: An overview
Production of tetrapyrrole compounds and vitamin B12 using genetically engineering of Propionibacterium freudenreichii.: An overview
复制标题
DOI：
10.1051/lait:2004035
复制
发表时间：
2005-01-01
期刊：
影响因子：
--
通讯作者：
Yamashita, M
中科院分区：
文献类型：
--
作者：
Murooka, Y;Piao, Y;Yamashita, M
费氏丙酸杆菌（Propionibacterium freudenreichii）是一种商业上重要的细菌，用于生产奶酪、钴胺素（维生素B-12）和丙酸。使用基因改良菌株的代谢工程将使发酵过程更经济，并提高产品质量。在丙酸杆菌中开发了使用来自费氏丙酸杆菌的强启动子的宿主载体系统和表达载体。通过使用这些表达载体和扩增各种基因，报道了5-氨基乙酰丙酸、四吡咯化合物和维生素B-12的生产。在此，我们回顾了近年来费氏原杆菌基因工程的进展，涵盖了四吡咯化合物和维生素B12形成的分子方面。
Propionibacterium freudenreichii is a commercially important bacterium that is used in the production of cheeses, cobalamin (vitamin B-12) and propionic acid. Metabolic engineering using genetically improved strains will make the fermentation process more economical and also enhance the quality of the products. Host-vector systems and expression vectors using strong promoters from P. freudenreichii were developed in propionibacteria. By using these expression vectors and amplification of various genes, productions of 5-aminolevulinic acid, tetrapyrrole compounds and vitamin B-12 were reported. Here, we review the advancement of genetic engineering in P. freudenreichii in recent years, covering the molecular aspects of the formation of tetrapyrrole compounds and vitamin B12.