Caspase-1 activation in macrophages infected with Yersinia pestis KIM requires the type III secretion system effector YopJ
Caspase-1 activation in macrophages infected with Yersinia pestis KIM requires the type III secretion system effector YopJ
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DOI：
10.1128/iai.01695-07
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发表时间：
2008-09-01
影响因子：
3.1
通讯作者：
Bliska, James B.
中科院分区：
文献类型：
--
作者：
Lilo, Sarit;Zheng, Ying;Bliska, James B.
Pathogenic Yersinia species utilize a type III secretion system (T3SS) to translocate effectors called Yersinia outer proteins (Yops) into infected host cells. Previous studies demonstrated a role for effector Yops in the inhibition of caspase-1-mediated cell death and secretion of interleukin-1 beta (IL-1 beta) in naive macrophages infected with Yersinia enterocolitica. Naive murine macrophages were infected with a panel of different Yersinia pestis and Yersinia pseudotuberculosis strains to determine whether Yops of these species inhibit caspase-1 activation. Cell death was measured by release of lactate dehydrogenase (LDH), and enzyme-linked immunosorbent assay for secreted IL-1 beta was used to measure caspase-1 activation. Surprisingly, isolates derived from the Y. pestis KIM strain (e. g., KIM5) displayed an unusual ability to activate caspase-1 and kill infected macrophages compared to other Y. pestis and Y. pseudotuberculosis strains tested. Secretion of IL-1 beta following KIM5 infection was reduced in caspase-1-deficient macrophages compared to wild-type macrophages. However, release of LDH was not reduced in caspase-1-deficient macrophages, indicating that cell death occurred independently of caspase-1. Analysis of KIM-derived strains defective for production of functional effector or translocator Yops indicated that translocation of catalytically active YopJ into macrophages was required for caspase-1 activation and cell death. Release of LDH and secretion of IL-1 beta were not reduced when actin polymerization was inhibited in KIM5-infected macrophages, indicating that extracellular bacteria translocating YopJ could trigger cell death and caspase-1 activation. This study uncovered a novel role for YopJ in the activation of caspase-1 in macrophages.
