A CONSERVED RETINA-SPECIFIC GENE ENCODES A BASIC MOTIF LEUCINE ZIPPER DOMAIN
A CONSERVED RETINA-SPECIFIC GENE ENCODES A BASIC MOTIF LEUCINE ZIPPER DOMAIN
复制标题
DOI：
10.1073/pnas.89.1.266
复制
发表时间：
1992-01-01
影响因子：
11.1
通讯作者：
AGARWAL, N
中科院分区：
文献类型：
--
作者：
SWAROOP, A;XU, JZ;AGARWAL, N
使用减法克隆，我们已经分离出一个人的cDNA，AS 321，这是在视网膜和视网膜母细胞瘤细胞系中表达，但没有在任何其他组织或细胞系测试。AS 321 mRNA在神经视网膜的所有细胞中检测到，在光感受器中高水平表达。从cDNA推导的多肽序列揭示了蛋白激酶A和脯氨酸指导的蛋白激酶的共识磷酸化位点。它的C-末端区域包含一个基本的基序和亮氨酸拉链结构域类似的DNA结合蛋白的jun和fos癌蛋白家族，它显示了很强的相似性，禽逆转录病毒癌基因，v-maf的产品。AS 321基因在进化过程中是保守的，并在脊椎动物视网膜中表达。我们建议将该基因命名为NRL（神经视网膜亮氨酸拉链）。
Using subtraction cloning, we have isolated a human cDNA, AS321, which is expressed in retina and retinoblastoma cell lines but not in any other tissue or cell line tested. AS321 mRNA is detected in all cells of neural retina, with a high level of expression in photoreceptors. The polypeptide sequence deduced from the cDNA reveals consensus phosphorylation sites for protein kinase A and proline-directed protein kinase. Its C-terminal region contains a basic motif and a leucine zipper domain similar to the DNA binding proteins of the jun and fos oncoprotein family, and it shows a strong similarity to the product of an avian retroviral oncogene, v-maf. The gene for AS321 is conserved during evolution and is expressed in vertebrate retina. We propose to name the gene NRL (neural retina leucine zipper).