Novel calcium phosphate ion-rechargeable and antibacterial adhesive to inhibit dental caries
Novel calcium phosphate ion-rechargeable and antibacterial adhesive to inhibit dental caries
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DOI：
10.1007/s00784-021-04002-7
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发表时间：
2021-06-10
影响因子：
3.4
通讯作者：
Xu, Hockin H. K.
中科院分区：
文献类型：
--
作者：
Al-Qarni, Faisal;Weir, Michael;Xu, Hockin H. K.
目的研制一种抗菌钙磷可充型粘接剂，并首次研究甲基丙烯酸二甲氨基十二烷基酯（DMAHDM）和纳米无定形磷酸钙（NACP）对牙本质粘接、生物膜反应、钙磷离子重复充放能力的影响。乙氧基化双酚A二甲基丙烯酸酯（EBPADMA）、甲基丙烯酸2-羟乙酯（HEMA）和双酚A二甲基丙烯酸缩水甘油酯（BisGMA）形成粘合剂（PEHB）。测试三组：（1）Scotchbond（SBMP，3 M）对照，（2）PEHB +30%NACP，和（3）PEHB +30%NACP +5%DMAHDM。测试样本的牙本质剪切粘结强度以及Ca和P离子释放、再充电和再释放。结果4组牙本质粘结强度无显著差异（p> 0.1），而3组牙本质粘结强度无显著差异（p > 0.1）。具有DMAHDM的粘合剂显示代谢活性、乳酸产生和生物膜CFU的显著降低（p < 0.05）。结论本研究开发了第一种钙磷离子可充电抗菌粘合剂，实现了较强的抗菌活性和钙磷离子的充电和再释放，可长期使用。
Objectives This study aimed to develop an antibacterial and calcium (Ca) and phosphate (P) rechargeable adhesive and investigate the effects of dimethylaminododecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP) on dentin bonding, biofilm response, and repeated Ca and P ion recharge and re-release capability for the first time.Materials and methods Pyromellitic glycerol dimethacrylate (PMGDM), ethoxylated bisphenol A dimethacrylate (EBPADMA), 2-hydroxyethyl methacrylate (HEMA), and bisphenol A glycidyl dimethacrylate (BisGMA) formed the adhesive (PEHB). Three groups were tested: (1) Scotchbond (SBMP, 3 M) control, (2) PEHB + 30% NACP, and (3) PEHB + 30% NACP + 5% DMAHDM. Specimens were tested for dentin shear bond strength, and Ca and P ion release, recharge, and re-release. Biofilm lactic acid production and colony-forming units (CFU) on resins were analyzed.Results The four groups had similar dentin shear bond strengths (p > 0.1). Adhesive with DMAHDM showed significant decrease in metabolic activity, lactic acid production, and biofilm CFU (p < 0.05). The adhesives containing NACP released high levels of Ca and P ions initially and after being recharged.Conclusion This study developed the first Ca and P ion-rechargeable and antibacterial adhesive, achieving strong antibacterial activity and Ca and P ion recharge and re-release for long-term remineralization.