Cartilage Tissue Engineering Application of Injectable Gelatin Hydrogel with In Situ Visible-Light-Activated Gelation Capability in Both Air and Aqueous Solution
Cartilage Tissue Engineering Application of Injectable Gelatin Hydrogel with In Situ Visible-Light-Activated Gelation Capability in Both Air and Aqueous Solution
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DOI：
10.1089/ten.tea.2013.0642
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发表时间：
2014-09-01
影响因子：
4.1
通讯作者：
Tuan, Rocky S.
中科院分区：
文献类型：
--
作者：
Lin, Hang;Cheng, Anthony Wai-Ming;Tuan, Rocky S.
软骨祖细胞包封在软骨支持性三维水凝胶支架中代表了一种有前途的关节软骨修复再生方法。在这项研究中，我们已经开发了一种可注射的，可生物降解的甲基丙烯酸化明胶（mGL）为基础的水凝胶能够通过可见光（VL）在空气或水溶液中激活交联快速凝胶化。温和的光交联条件允许在凝胶化过程中掺入细胞。包封的人骨髓间充质干细胞（hBMSCs）在整个支架中表现出高的长期存活力（长达90天）。为了评估mGL水凝胶用于软骨组织工程的适用性，我们使用接种在琼脂糖中的hBMSCs作为对照，评估了包封的hBMSCs的软骨形成的功效。利用体外软骨修复模型进一步研究了负载hBMSC的mGL构建体在植入后与宿主组织整合的能力。结果表明，mGL水凝胶，它可以在空气和水溶液中光聚合，支持hBMSC生长和TGF-β 3诱导的软骨形成。与琼脂糖相比，装载有hBMSC的mGL构建体随着时间的推移机械强度更强，并且基于推出机械测试在植入后与天然软骨组织很好地整合。VL-光交联的mGL支架因此代表了用于关节软骨缺损的基于细胞的修复和表面重建的有前景的支架。
Chondroprogenitor cells encapsulated in a chondrogenically supportive, three-dimensional hydrogel scaffold represents a promising, regenerative approach to articular cartilage repair. In this study, we have developed an injectable, biodegradable methacrylated gelatin (mGL)-based hydrogel capable of rapid gelation via visible light (VL)-activated crosslinking in air or aqueous solution. The mild photocrosslinking conditions permitted the incorporation of cells during the gelation process. Encapsulated human-bone-marrow-derived mesenchymal stem cells (hBMSCs) showed high, long-term viability (up to 90 days) throughout the scaffold. To assess the applicability of the mGL hydrogel for cartilage tissue engineering, we have evaluated the efficacy of chondrogenesis of the encapsulated hBMSCs, using hBMSCs seeded in agarose as control. The ability of hBMSC-laden mGL constructs to integrate with host tissues after implantation was further investigated utilizing an in vitro cartilage repair model. The results showed that the mGL hydrogel, which could be photopolymerized in air and aqueous solution, supports hBMSC growth and TGF-beta 3-induced chondrogenesis. Compared with agarose, mGL constructs laden with hBMSCs are mechanically stronger with time, and integrate well with native cartilage tissue upon implantation based on push-out mechanical testing. VL-photocrosslinked mGL scaffold thus represents a promising scaffold for cell-based repair and resurfacing of articular cartilage defects.