Anti-inflammatory effect of lysozyme from hen egg white on mouse peritoneal macrophages
Anti-inflammatory effect of lysozyme from hen egg white on mouse peritoneal macrophages
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DOI：
10.1007/s10616-017-0184-2
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发表时间：
2018-03
期刊：
影响因子：
2.2
通讯作者：
Ayuka Tagashira;K. Nishi;S. Matsumoto;T. Sugahara
中科院分区：
文献类型：
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作者：
Ayuka Tagashira;K. Nishi;S. Matsumoto;T. Sugahara
据报道，来自鸡蛋的溶菌酶具有抗炎作用。然而，对其详细机制知之甚少。本文对溶菌酶的抗炎作用机制进行了初步探讨。用脂多糖（LPS）激活小鼠巨噬细胞样细胞系RAW264.7细胞和小鼠腹腔巨噬细胞，再用溶菌酶处理后，肿瘤坏死因子-α和白细胞介素-6的产生明显受到抑制。该效应通过抑制两种细胞因子的基因表达水平来诱导。腹腔巨噬细胞的吞噬活性不受溶菌酶处理的影响，表明溶菌酶显示抗炎作用而不抑制巨噬细胞的吞噬反应。此外，溶菌酶抑制c-jun N-末端激酶（JNK）的磷酸化，并在用溶菌酶处理细胞后1小时内被巨噬细胞吸收。总体结果表明，溶菌酶被细胞内摄取，并通过抑制JNK磷酸化来抑制LPS诱导的炎症反应。
Lysozyme from hen egg has been reported to possess an anti-inflammatory effect. However, little is known about its detailed mechanism. The mechanism of anti-inflammatory effect of lysozyme was examined in this study. When mouse macrophage-like cell line RAW264.7 cells and mouse peritoneal macrophages were activated with lipopolysaccharide (LPS) and then treated with lysozyme, the production of tumor necrosis factor-α and interleukin-6 was significantly suppressed. The effect was induced by suppressing the gene expression levels of both cytokines. Phagocytosis activity of peritoneal macrophages was not altered by the treatment with lysozyme, suggesting that lysozyme shows the anti-inflammatory effect without inhibiting the phagocytotic response of macrophages. In addition, lysozyme inhibited phosphorylation of c-jun N-terminal kinase (JNK) and was taken up by macrophages within 1 h after treatment of the cells with lysozyme. Overall results suggest that lysozyme is taken up intracellularly and suppresses LPS-induced inflammatory responses by inhibiting JNK phosphorylation.