Reduction of pulmonary compliance found with high-resolution computed tomography in irradiated mice
Reduction of pulmonary compliance found with high-resolution computed tomography in irradiated mice
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DOI：
10.1016/j.ijrobp.2006.10.037
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发表时间：
2007-03-01
影响因子：
7
通讯作者：
Travis, Elizabeth
中科院分区：
文献类型：
--
作者：
Guerrero, Thomas;Castillo, Richard;Travis, Elizabeth
目的：证明高分辨率计算机断层扫描 (CT) 可用于量化受辐射小鼠的肺顺应性损失。方法和材料：在单剂量 16-Gy Co (60) 胸部辐射后 200 天，获得三只未受辐射（对照）和三只受辐射小鼠的计算机断层扫描图像。插管时，每只动物在 2、10 和 18 cm H2O 的静态屏气压力下进行成像。使用可变形图像配准算法来计算相邻压力 CT 图像对（例如 2 和 10 cm H2O）之间的空气量变化，并生成肺顺应性的功能图像。质量特异性顺应性计算为体积变化除以 2 个图像集和肺组织质量之间的压力差。结果：对于受辐射的小鼠，肺实质平均 CT 值范围为 -314 (+/- 11) Hounsfield 单位 (HU) 到 -378 (+/- 11) HU。对于对照小鼠，平均 CT 值范围为 -549 (:L 11) HU 至 -633 (+/- 11) HU。从 2 厘米到 10 厘米水柱 CT 图像对来看，受辐射小鼠的质量特异性顺应性比对照组低 60%（45%、74%；95% 置信区间）（0.039 [+/- 0.0038] vs. 0.106 [+/- 0.0038] mL 空气/cm H2O/g 肺）。在较高的膨胀压力下，各组之间的顺应性差异不太明显。结论：高分辨率 CT 用于量化小鼠全肺照射后肿块特异性顺应性的减少。这种小动物辐射损伤模型和测定可能有助于肺损伤的研究。 (c) 2007 爱思唯尔公司
Purpose: To demonstrate that high-resolution computed tomography (CT) can be used to quantify loss of pulmonary compliance in irradiated mice.Methods and Materials: Computed tomography images of three nonirradiated (controls) and three irradiated mice were obtained 200 days after a single dose of 16-Gy Co (60) thoracic irradiation. While intubated, each animal was imaged at static breath-hold pressures of 2, 10, and 18 cm H2O. A deformable image registration algorithm was used to calculate changes in air volume between adjacent-pressure CT image pairs (e.g., 2 and 10 cm H2O), and functional images of pulmonary compliance were generated. The mass-specific compliance was calculated as the change in volume divided by the pressure difference between the 2 image sets and the mass of lung tissue.Results: For the irradiated mice, the lung parenchyma mean CT values ranged from -314 (+/- 11) Hounsfield units (HU) to -378 (+/- 11) HU. For the control mice, the mean CT values ranged from -549 (:L 11) HU to -633 (+/- 11) HU. Irradiated mice had a 60% (45, 74%; 95% confidence interval) lower mass-specific compliance than did the controls (0.039 [+/- 0.0038] vs. 0.106 [+/- 0.0038] mL air per cm H2O per g lung) from the 2-cm to 10-cm H2O CT image pair. The difference in compliance between groups was less pronounced at the higher distending pressures.Conclusion: High-resolution CT was used to quantify a reduction in mass-specific compliance following whole lung irradiation in mice. This small animal radiation injury model and assay may be useful in the study of lung injury. (c) 2007 Elsevier Inc.