Common skin bacteria protect their host from oxidative stress through secreted antioxidant RoxP
Common skin bacteria protect their host from oxidative stress through secreted antioxidant RoxP
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DOI：
10.1038/s41598-019-40471-3
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发表时间：
2019-03-05
影响因子：
4.6
通讯作者：
Lood, Rolf
中科院分区：
文献类型：
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作者：
Andersson, Tilde;Bergdahl, Gizem Erturk;Lood, Rolf
痤疮表皮杆菌是一种丰富的皮肤共生菌，具有几种相互作用。最近从痤疮C.分泌组中鉴定出一种具有较强抗氧化活性的蛋白。这种蛋白质被称为RoxP，促进有氧细菌在体外和体外的生长。由于还原事件自然发生在细菌细胞外，因此进一步假设RoxP也可以调节人体皮肤的氧化还原状态。在这里，通过氧化应激细胞培养和氧化性疾病（光化性角化病和基底细胞癌）影响皮肤的蛋白质定量，分别在体外和体内评估RoxP的生物学功能。使用16S rDNA扩增子深度测序和单位点序列分型将细菌患病率与皮肤RoxP丰度联系起来。我们发现RoxP对暴露于氧化应激下的单核细胞和角质形成细胞的活力有积极影响，并且在受氧化疾病影响的皮肤中可以观察到RoxP浓度的一致下降。此外，基底细胞癌与微生物生态失调有关，其特征是痤疮杆菌患病率降低。痤疮C.痤疮分泌的RoxP是一种外源性但天然存在的抗氧化剂，可能对人类宿主产生积极影响。结果进一步证明了其作为生物制药的潜在可用性。
Cutibacterium acnes is an abundant skin commensal with several proposed mutualistic functions. A protein with strong antioxidant activity was recently identified from the C. acnes secretome. This protein, termed RoxP, facilitated aerobic bacterial growth in vitro and ex vivo. As reducing events naturally occurred outside of the bacterial cell, it was further hypothesized that RoxP could also serve to modulate redox status of human skin. The biological function of RoxP was here assessed in vitro and in vivo, through oxidatively stressed cell cultures and through protein quantification from skin affected by oxidative disease (actinic keratosis and basal cell carcinoma), respectively. 16S rDNA amplicon deep sequencing and single locus sequence typing was used to correlate bacterial prevalence to cutaneous RoxP abundances. We show that RoxP positively influence the viability of monocytes and keratinocytes exposed to oxidative stress, and that a congruent concentration decline of RoxP can be observed in skin affected by oxidative disease. Basal cell carcinoma was moreover associated with microbial dysbiosis, characterized by reduced C. acnes prevalence. C. acnes's secretion of RoxP, an exogenous but naturally occurring antioxidant on human skin, is likely to positively influence the human host. Results furthermore attest to its prospective usability as a biopharmaceutical.