'Candidatus Phytoplasma ziziphi', a novel phytoplasma taxon associated with jujube witches'-broom disease
'Candidatus Phytoplasma ziziphi', a novel phytoplasma taxon associated with jujube witches'-broom disease
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DOI：
10.1099/ijs.0.02393-0
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发表时间：
2003-07-01
影响因子：
2.8
通讯作者：
Namba, S
中科院分区：
文献类型：
--
作者：
Jung, HY;Sawayanagi, T;Namba, S
采用16S rDNA聚合酶链式反应(16S RDNA)扩增和序列分析方法，对来自4个不同地区的5个枣疯病植原体分离物与其他植原体的亲缘关系进行了研究。JWB植原体五个分离物中任意一对的16S rDNA序列同源性为99.5%。JWB植原体的16S rDNA序列与榆树黄化植原体的16S rDNA序列的亲缘关系最近。JWB植原体的16S rDNA序列与GenBank中收录的大多数植原体的16S rDNA序列进行了系统发育分析，形成了JWB的系统发育树，其中JWB分离物作为一个独立的亚群聚在一起。JWB植原体的独特性似乎与特定的昆虫媒介(Hishimonus Sellatus)和寄主植物(Zizyphus Jujuba)有关，或与特定的地理分布有关。JWB植原体序列的独特性质清楚地表明，它代表了一个新的分类单元，‘Candidatus pltodium ziziphi’。
Phylogenetic relationships of five jujube witches'-broom (JWB) phytoplasma isolates from four different districts, and other phytoplasmas, were investigated by 16S rDNA PCR amplification and sequence analysis. The 16S rDNA sequences of any pair of the five isolates of JWB phytoplasmas were >99.5% similar. The JWB phytoplasma 16S rDNA sequences were most closely related to that of the elm yellows (EY) phytoplasma in 16S-group VIII. Phylogenetic analysis of the 16S rDNA sequences from the JWB phytoplasma isolates, together with sequences from most of the phytoplasmas archived in GenBank, produced a tree in which the JWB isolates clustered as a discrete subgroup. The uniqueness of the JWB phytoplasma appears to be correlated with a specific insect vector (Hishimonus sellatus) and the host plant (Zizyphus jujuba), or with a specific geographical distribution. The unique properties of the JWB phytoplasma sequences clearly indicate that it represents a novel taxon, 'Candidatus Phytoplasma ziziphi'.