Prevalence of Shiga toxin-producing Escherichia coli, Salmonella spp. and Campylobacter spp. in large game animals intended for consumption: Relationship with management practices and livestock influence
Prevalence of Shiga toxin-producing Escherichia coli, Salmonella spp. and Campylobacter spp. in large game animals intended for consumption: Relationship with management practices and livestock influence
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DOI：
10.1016/j.vetmic.2012.12.026
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发表时间：
2013-05-03
影响因子：
3.3
通讯作者：
Vidal, D.
中科院分区：
文献类型：
--
作者：
Diaz-Sanchez, S.;Sanchez, S.;Vidal, D.
虽然野生反刍动物已被确定为产志贺毒素大肠杆菌（STEC）的水库，很少有资料是关于沙门氏菌的作用。和弯曲杆菌属（Campylobacter spp.）在大型猎物中。我们评估了这些病原体的存在下，粪便（N = 574）和尸体（N = 585）采样红鹿（N = 295），野猪（N = 333）和其他有蹄类动物（休闲鹿，毛羚）（N = 9）。在两个狩猎季节，从33个狩猎庄园现场进行动物采样。沙门氏菌和弯曲杆菌属（Campylobacter spp.）与人类弯曲杆菌病相关的菌株很少被检测到，表明这两种病原体在我们的研究区域具有有限的人畜共患病风险。动物中STEC的总体患病率为21%（134/637），红鹿粪便中的患病率显著更高（90/264）。共对58株分离株进行了血清分型。血清型O 146：H-和O27：H30在红鹿中最常见，并且来自红鹿和野猪的大多数分离株来自先前在与人类感染相关的STEC菌株中发现的血清型，包括血清型O 157：H7。红鹿粪便中STEC的患病率在存在牲畜的情况下显著更高（p <0.01），其中存在高密度的红鹿（p < 0.001）。据我们所知，这是第一项报告沙门氏菌发生的研究。以及大型狩猎动物尸体中的STEC。此外，本研究通过脉冲场凝胶电泳（PFGE）证实，在屠宰过程中发生了STEC交叉污染，这意味着这些病原体可能进入食物链。(C)2013爱思唯尔有限公司版权所有。
Although wild ruminants have been identified as reservoirs of Shiga-toxin producing Escherichia coli (STEC), little information is available concerning the role of Salmonella spp. and Campylobacter spp. in large game species. We evaluated the presence of these pathogens in faeces (N = 574) and carcasses (N = 585) sampled from red deer (N = 295), wild boar (N = 333) and other ungulates (fallow deer, mouflon) (N = 9). Animal sampling was done in situ from 33 hunting estates during two hunting seasons. Salmonella spp. and Campylobacter spp. strains associated with human campylobacteriosis were infrequently detected indicating that both pathogens had a limited zoonotic risk in our study area. The overall STEC prevalence in animals was 21% (134/637), being significantly higher in faeces from red deer (90 out of 264). A total of 58 isolates were serotyped. Serotypes O146:H- and O27:H30 were the most frequent in red deer and the majority of isolates from red deer and wild boar were from serotypes previously found in STEC strains associated with human infection, including the serotype O157:H7. The STEC prevalence in red deer faeces was significantly higher with the presence of livestock (p < 0, 01) where high densities of red deer (p < 0.001) were present. To the best of our knowledge, this is the first study reporting the occurrence of Salmonella spp. and STEC in carcasses of large game animals. Furthermore, this study confirmed by pulsed-field gel electrophoresis (PFGE) that cross contamination of STEC during carcass dressing occurred, implying the likelihood of these pathogens entering into the food chain. (C) 2013 Elsevier B.V. All rights reserved.