The localization of inner centromeric protein (INCENP) at the cleavage furrow is dependent on Kif12 and involves interactions of the N terminus of INCENP with the actin cytoskeleton
The localization of inner centromeric protein (INCENP) at the cleavage furrow is dependent on Kif12 and involves interactions of the N terminus of INCENP with the actin cytoskeleton
复制标题
DOI：
10.1091/mbc.e06-10-0895
复制
发表时间：
2007-09-01
影响因子：
3.3
通讯作者：
De Lozanne, Arturo
中科院分区：
文献类型：
--
作者：
Chen, Qian;Lakshmikanth, Gandikota S.;De Lozanne, Arturo
已知内着丝粒蛋白（INCENP）和其他染色体乘客蛋白定位于卵裂沟并在胞质分裂中起作用。然而，目前尚不清楚INCENP如何定位在犁沟上，或者这种定位是否可以与中体处的定位分开。在这里，我们表明，该协会的Dictyosteoprotein INCENP（DdINCENP）与皮层的分裂沟涉及与肌动蛋白细胞骨架的相互作用，并依赖于驱动蛋白-6相关蛋白Kif 12的存在。我们发现Kif 12在胞质分裂过程中被发现在中央纺锤体和卵裂沟上。DdINCENP从着丝粒到纺锤体的再分配不需要Kif 12。然而，在没有Kif 12的情况下，DdINCENP不能定位在卵裂沟上。结构域分析表明，N端的DdINCENP沟定位是必要的和足够的，它直接结合到肌动蛋白细胞骨架。我们的数据表明，INCENP从中央纺锤体的分裂细胞的沟Kif 12依赖的途径。一旦INCENP到达赤道皮质，它与肌动蛋白细胞骨架结合，然后集中在胞质分裂的末端。
The inner centromeric protein (INCENP) and other chromosomal passenger proteins are known to localize on the cleavage furrow and to play a role in cytokinesis. However, it is not known how INCENP localizes on the furrow or whether this localization is separable from that at the midbody. Here, we show that the association of Dictyostelium INCENP (DdINCENP) with the cortex of the cleavage furrow involves interactions with the actin cytoskeleton and depends on the presence of the kinesin-6-related protein Kif12. We found that Kif12 is found on the central spindle and the cleavage furrow during cytokinesis. Kif12 is not required for the redistribution of DdINCENP from centromeres to the central spindle. However, in the absence of Kif12, DdINCENP fails to localize on the cleavage furrow. Domain analysis indicates that the N terminus of DdINCENP is necessary and sufficient for furrow localization and that it binds directly to the actin cytoskeleton. Our data suggest that INCENP moves from the central spindle to the furrow of a dividing cell by a Kif12-dependent pathway. Once INCENP reaches the equatorial cortex, it associates with the actin cytoskeleton where it then concentrates toward the end of cytokinesis.