SEQUENCE OF RNA SEGMENT-7 OF THE INFLUENZA-B VIRUS GENOME - PARTIAL AMINO-ACID HOMOLOGY BETWEEN THE MEMBRANE PROTEINS-(M1) OF INFLUENZA-A AND INFLUENZA-B VIRUSES AND CONSERVATION OF A 2ND OPEN READING FRAME
SEQUENCE OF RNA SEGMENT-7 OF THE INFLUENZA-B VIRUS GENOME - PARTIAL AMINO-ACID HOMOLOGY BETWEEN THE MEMBRANE PROTEINS-(M1) OF INFLUENZA-A AND INFLUENZA-B VIRUSES AND CONSERVATION OF A 2ND OPEN READING FRAME
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DOI：
10.1016/0042-6822(82)90150-7
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发表时间：
1982-01-01
期刊：
影响因子：
3.7
通讯作者：
CHOPPIN, PW
中科院分区：
文献类型：
--
作者：
BRIEDIS, DJ;LAMB, RA;CHOPPIN, PW
测定了乙型流感/Lee/40病毒基因组RNA第7片段的一个克隆全长DNA拷贝的完整核苷酸序列。该病毒非糖基化膜蛋白（M1）的mRNA（约1175个病毒核苷酸）能够编码一个含248个氨基酸的蛋白质，其中63个氨基酸在甲型和乙型流感病毒之间是保守的。一个内部疏水结构域，在相同位置包含3个半胱氨酸残基，在这两种病毒的M1蛋白之间也是保守的，可能具有功能意义。第二个编码区与乙型流感M1蛋白的编码区有86个氨基酸重叠。根据mRNA是否剪接以及起始甲硫氨酸残基的位置，这个区域在+2阅读框中最多可编码195个氨基酸。这个区域可能编码一种类似于甲型流感病毒M2蛋白的蛋白质。在这两种病毒的M2蛋白推导的氨基酸序列中未检测到明显的同源性。甲型和乙型流感病毒之间M1编码区的大小是保守的，乙型病毒第7片段更大，差异主要是由于M2编码区更长。
The complete nucleotide sequence of a cloned full-length DNA copy of genome RNA segment 7 of influenza B/Lee/40 virus was determined. The mRNA (.apprx. 1175 viral nucleotides) for the nonglycosylated membranes protein of the virus (M1) is capable of coding for a protein of 248 amino acids, of which 63 are conserved between influenza A and B viruses. An internal hydrophobic domain, which includes 3 cysteine residues in the same positions, is also conserved between the M1 proteins of the 2 viruses and may be of functional significance. A 2nd coding region overlaps that of the influenza B M1 protein by 86 amino acids. This region could code for a maximum of 195 amino acids in the +2 reading frame, depending on whether or not the mRNA is spliced and the location of the initiating methionine residue. This region is likely to code for a protein analogous to the influenza A virus M2 protein. No obvious homology was detected in the deduced amino sequences of the M2 proteins of the 2 viruses. The size of the M1 coding regions is conserved between influenza A and B viruses, the B virus segment 7 is larger, with the difference largely due to a longer M2 coding region.