Analysis of Tear Cytokines and Clinical Correlations in Sjogren Syndrome Dry Eye Patients and Non-Sjogren Syndrome Dry Eye Patients
Analysis of Tear Cytokines and Clinical Correlations in Sjogren Syndrome Dry Eye Patients and Non-Sjogren Syndrome Dry Eye Patients
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DOI：
10.1016/j.ajo.2013.04.003
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发表时间：
2013-08-01
影响因子：
4.2
通讯作者：
Seo, Kyoung Yul
中科院分区：
文献类型：
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作者：
Lee, Sang Yeop;Han, Soo Jung;Seo, Kyoung Yul
目得：比较干燥综合征（SS）干眼、非干燥综合征（non-SS）干眼和正常人3组泪液细胞因子的浓度。眼表参数和泪液细胞因子之间的相关性也investigated.DESIGN：前瞻性横断面研究。METHODS：SS干眼症患者（n = 24，40眼）被诊断为原发性SS根据美国-欧洲共识组设置的标准。非SS干眼患者（n = 25; 40只眼）和正常受试者（n = 21; 35只眼）也入组。通过多重免疫珠测定法测量白细胞介素（IL）-17，IL-6，IL-10，IL-4，IL-2，干扰素γ（IFN-γ）和肿瘤坏死因子α（TNF-α）的泪液浓度。眼表疾病指数（OSDI），泪膜破裂时间（TBUT），Schirmer I试验，和荧光素染色scores从干眼症patients.Results：所有细胞因子水平，除了IL-2是最高的SS组，其次是非SS干眼症组和对照组。IL-17、TNF-α和IL-6的浓度显著升高。3组间差异（IL-17：SS>对照P <.001，非SS>对照P =.042，SS>非SS P <.001; TNF-α：SS>对照P =.006，非SS>对照P =.034，SS>非SS P =.029; IL-6：SS>对照P =.001，非SS>对照P =.001，TNF-α：SS>对照P =.006，非SS>对照P =.034，SS>非SS P =.029; IL-17：SS>对照P =.001，非SS>对照P =.042，SS>非SS P =.001; TNF-α：SS>对照P =.006，非SS>对照P =.034，SS>非SS P =.029; IL-6：SS>对照P =.0001，非SS>对照P =.0001，IL-17：SS>对照P =.0001 SS>对照P =.002，非SS>对照P =.032，SS>非SS P =.002）。SS组IL-17与TBUT（R = 0.22，P = 0.012）和Schirmer I试验（R =-0.36，P =0.027）评分显著相关。IL-6仅与非SS组的TBUT显著相关（R =-0.38，P =0.02）。结论：SS干眼症和非SS干眼症患者泪液细胞因子水平和相关模式的差异提示干眼综合征的病因涉及不同的炎症过程。(C)2013年，Elsevier Inc. All rights reserved.
PURPOSE: To compare concentrations of tear cytokines in 3 groups composed of Sjogren syndrome (SS) dry eye, non-Sjogren syndrome (non-SS) dry eye, and normal subjects. Correlations between ocular surface parameters and tear cytokines were also investigated.DESIGN: Prospective cross-sectional study.METHODS: SS dry eye patients (n = 24; 40 eyes) were diagnosed with primary SS according to the criteria set by the American-European Consensus Group. Non-SS dry eye patients (n = 25; 40 eyes) and normal subjects (n = 21; 35 eyes) were also enrolled. Tear concentrations of interleukin (IL)-17, IL-6, IL-10, IL-4, IL-2, interferon gamma (IFN-gamma), and tumor necrosis factor alpha (TNF-alpha) were measured by a multiplex immunobead assay. Ocular Surface Disease Index (OSDI), tear film breakup time (TBUT), Schirmer I test, and fluorescein staining scores were obtained from dry eye patients.RESULTS: All cytokine levels except for IL-2 were highest in the SS group, followed by non-SS dry eye group and control subjects. Concentrations of IL-17, TNF-alpha, and IL-6 were significantly. different among the 3 groups (IL-17: SS > control P < .001, non-SS > control P = .042, SS > non-SS P < .001; TNF-alpha: SS > control P = .006, non-SS > control P = .034, SS > non-SS P = .029; IL-6: SS > control P = .002, non-SS > control P = .032, SS > non-SS P = .002). IL-17 was significantly correlated with TBUT (R = 0.22, P = .012) and Schirmer I test (R = -0.36, P = .027) scores in the SS group. IL-6 was significantly correlated only with TBUT (R = -0.38, P = .02) in the non-SS group.CONCLUSIONS: Differences in tear cytokine levels and correlation patterns between SS dry eye and non-SS dry eye patients suggest the involvement of different inflammatory processes as causes of dry eye syndrome. (C) 2013 by Elsevier Inc. All rights reserved.