DECREASED UPTAKE AND RETENTION OF RHODAMINE-123 BY MITOCHONDRIA IN FELINE SARCOMA VIRUS-TRANSFORMED MINK CELLS
DECREASED UPTAKE AND RETENTION OF RHODAMINE-123 BY MITOCHONDRIA IN FELINE SARCOMA VIRUS-TRANSFORMED MINK CELLS
复制标题
DOI：
10.1016/0092-8674(82)90369-5
复制
发表时间：
1982-01-01
期刊：
影响因子：
64.5
通讯作者：
CHEN, LB
中科院分区：
文献类型：
--
作者：
JOHNSON, LV;SUMMERHAYES, IC;CHEN, LB
检测到猫肉瘤病毒（FeSV）转化水貂成纤维细胞（64F3）对线粒体特异性膜电位探针罗丹明123的摄取和保留减少。64F3线粒体罗丹明123积累的减少不是由于质膜染料渗透性异常，因为在显微注射染料后，这些细胞仍然无法将染料保持在与未转化的亲本细胞CCL 64相当的水平。尼日利亚素是一种离子载体，介导K离子的电中性质子交换，导致线粒体膜上pH梯度（. delta .pH）的消除，线粒体膜电位随着持续呼吸的代偿性增加，增加了转化64F3细胞中染料的摄取和保留时间。因此，在fesv转化的水貂细胞中，线粒体可能具有异常低的线粒体膜电位，并伴有相对的δ - ph。因为阴离子代谢物如丙酮酸和谷氨酸是由线粒体按比例累积的。线粒体膜的pH值，此处描述的异常线粒体可能导致fesv转化细胞的异常代谢状态。
A reduced uptake and retention of the mitochondrial-specific membrane potential probe rhodamine 123 by feline sarcoma virus (FeSV)-transformed mink fibroblasts (64F3) was detected. The decreased accumulation of rhodamine 123 by 64F3 mitochondria is not due to abnormal plasma membrane dye permeability, since after microinjection of the dye these cells are still unable to retain the dye at levels comparable to the untransformed parental cells, CCL 64. Nigericin, an ionophore that mediates an electrically neutral exchange of protons for K ions, resulting in the elimination of the pH gradient (.DELTA.pH) across the mitochondrial membrane and a compensatory increase in mitochondrial membrane potential with continued respiration, increases both the dye uptake and the retention time in transformed 64F3 cells. Thus mitochondria in FeSV-transformed mink cells may have an abnormally low mitochondrial membrane potential accompanied by a relatively .DELTA.pH. Since anionic metabolites such as pyruvate and glutamate are accumulated by mitochondria in proportion to the .DELTA.pH across the mitochondrial membrane, the abnormal mitochondria described here may contribute to the abnormal metabolic state of FeSV-transformed cells.