Assessment of genetic diversity in selected breeding lines and cultivars of canola quality Brassica juncea and their implications for canola breeding
Assessment of genetic diversity in selected breeding lines and cultivars of canola quality Brassica juncea and their implications for canola breeding
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DOI：
10.1023/b:euph.0000030672.56206.f0
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发表时间：
2004-01-01
期刊：
影响因子：
1.9
通讯作者：
Salisbury, PA
中科院分区：
文献类型：
--
作者：
Burton, WA;Ripley, VL;Salisbury, PA
利用AFLP标记对来自加拿大(加拿大农业和农业食品公司和萨斯喀彻温省小麦池)和澳大利亚(维多利亚农业公司)的三个世界主要油菜优质芥菜育种项目的77个育种系的遗传多样性进行了评估。本文的目的是评估这三个育种计划内部和之间的遗传多样性，并评估油菜优质种质与芥菜优质芥菜的遗传多样性。调查还包括来自印度、中国、俄罗斯和澳大利亚的15个芥菜品质品系。以EcoR1/Mse1为基础的10对引物共扩增出751条可评分片段，平均每对扩增出26条多态性条带(35%)。利用简单匹配系数计算相似系数，利用UPGMA程序绘制系统树，将种质划分为5个主要类群。利用加速回交等育种技术，发现了有潜力提高个体育种计划效率的品系特异标记。进一步了解项目内和项目间的遗传多样性对未来的育种和三个项目内和项目之间的合作具有重要意义。
AFLP markers were used to assess the genetic diversity of 77 breeding lines from three of the world's major canola quality Brassica juncea breeding programs from Canada (Agriculture and Agri-Food Canada and Saskatchewan Wheat Pool) and Australia (Agriculture Victoria). The objectives of the paper were to assess the genetic diversity within and between these three breeding programs and to assess genetic diversity of the canola quality germplasm as compared to mustard quality B. juncea. Fifteen lines of mustard quality B. juncea from India, China, Russia and Australia were also included in the investigation. Ten EcoR1/Mse1 based primer pairs generated 751 scorable fragments with an average of 26 polymorphic bands per primer pair (35%). Similarity coefficients were calculated using the Simple Matching coefficient and a dendrogram was developed using the UPGMA procedure, resulting in germplasm being partitioned into five main groups. Line specific markers were discovered that have potential in enhancing the efficiency of individual breeding programs using breeding techniques like accelerated backcrossing. Further understanding the genetic diversity within and between programs has implications for future breeding and collaboration within and between the three programs.