Surface-enhanced Raman spectroscopy (SERS) investigations of saliva for oral cancer diagnosis
Surface-enhanced Raman spectroscopy (SERS) investigations of saliva for oral cancer diagnosis
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DOI：
10.1007/s10103-020-02988-2
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发表时间：
2020-08-01
影响因子：
2.1
通讯作者：
Hedesiu, M.
中科院分区：
文献类型：
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作者：
Falamas, A.;Rotaru, H.;Hedesiu, M.
唾液可能是非侵入性癌症检测的最佳样本，因为它含有大量的蛋白质和代谢物，可以反映个体的健康状况。此外，将其与高灵敏度的无标记检测技术相结合，可以成功地用于早期癌症诊断。在这项研究中，我们探索的唾液特征拉曼带的增强，使用无标记，超灵敏的表面增强拉曼散射（Sers）的基础上的金纳米粒子。Sers图谱从与Au胶体纳米颗粒混合的唾液上清液的干燥样品中获得，然后将其移液到干净的载玻片上。Sers光谱呈现出信号强度和频率偏移的高度可变性。然而，在图上的某些位置处获得了显示良好分辨的条带的几个可再现的Sers光谱，其中Au纳米颗粒在空气干燥期间聚集在一起。利用主成分分析法可以区分健康唾液和口腔癌唾液，该方法基于几个主要分配给氨基酸和蛋白质的Sers条带。此外，硫氰酸盐拉曼模式中检测到的吸烟和不吸烟的志愿者和癌症患者的唾液样品。分析表明，癌症组显示出总体上较高水平的2126 cm（-1）带面积分配给硫氰酸盐的C-N伸缩振动。
Saliva could be an optimal sample for non-invasive cancer detection, as it contains plenty of proteins and metabolites which can reflect the health status of an individual. Moreover, pairing it with high-sensitivity, label-free detection techniques could prove successful for early cancer diagnosis. In this study, we explore the enhancement of salivary characteristic Raman bands by using label-free, ultrasensitive surface-enhanced Raman scattering (SERS) based on gold nanoparticles. SERS maps were acquired from dry samples of saliva supernatant mixed with Au colloidal nanoparticles, which was then pipetted on clean glass slides. The SERS spectra presented a high variability of signal intensities and frequency shifts. However, several reproducible SERS spectra showing well-resolved bands were obtained at certain locations on the maps, where Au nanoparticles clustered together during the air-drying. The healthy and oral cancer saliva could be differentiated using principal components analysis based on several SERS bands assigned mainly to amino acids and proteins. Moreover, thiocyanate Raman modes were detected in saliva samples of both smoking and non-smoking volunteers and cancer patients. The analysis indicated that the cancer group displayed an overall higher level of the 2126 cm(-1)band area assigned to C-N stretching vibrations of thiocyanate.