EXPRESSION OF C-FOS-LIKE PROTEIN AS A MARKER FOR NEURONAL-ACTIVITY FOLLOWING NOXIOUS-STIMULATION IN THE RAT
EXPRESSION OF C-FOS-LIKE PROTEIN AS A MARKER FOR NEURONAL-ACTIVITY FOLLOWING NOXIOUS-STIMULATION IN THE RAT
复制标题
DOI：
10.1002/cne.902960402
复制
发表时间：
1990-06-22
影响因子：
2.5
通讯作者：
BULLITT, E
中科院分区：
文献类型：
--
作者：
BULLITT, E
C-fos是一种原癌基因，在去极化后在一些神经元内表达。蛋白质产物c-fos蛋白可通过免疫组织化学技术鉴定。因此，c-fos的表达可能被用来作为一个标记的神经元活动后，外周刺激整个轴突。这项研究分析了对照动物和麻醉动物以及受到各种形式外周刺激的麻醉大鼠中c-fos的表达模式。对脊髓、脑干、下丘脑和丘脑中标记的细胞进行计数。在对照组动物中几乎没有c-fos免疫反应。长时间吸入麻醉增加了标记细胞的数量在几个脑干网站。麻醉大鼠的noclonal刺激诱导c-fos的神经轴内的模式与从电生理学研究中获得的数据一致，并在其他位置，其中一些直接的电生理数据是可用的，如腹外侧延髓，下丘脑后核，和reuniens和室旁丘脑核。温和的机械刺激对诱导c-fos样蛋白无效。这些数据表明，c-fos可以被用作伤害性刺激后神经元活动的跨突触标记。然而，c-fos仅在某些类型的神经元中表达，因此它可能是一个不完整的标记伤害反应活动。此外，在没有伤害性刺激的情况下，至少有少数神经元表达c-fos蛋白。分析c-fos表达的实验必须小心设计，因为外来刺激和麻醉深度都会影响结果。
C-fos is a proto-oncogene that is expressed within some neurons following depolarization. The protein product, c-fos protein, can be identified by immunohistochemical techniques. Therefore, c-fos expression might be used as a marker for neuronal activity throughout the neuraxis following peripheral stimulation. This study has analyzed patterns of c-fos expression in both control and anesthetized animals and in anesthetized rats subjected to various forms of peripheral stimulation. Labeled cells were counted in the spinal cord, brainstem, hypothalamus, and thalamus. Little c-fos immunoreactivity was found in control animals. Prolonged inhalational anesthesia increased the number of labeled cells at several brainstem sites. Noxious stimulation of anesthetized rats induced c-fos within the neuraxis in patterns consistent with data obtained from electrophysiological studies and in additional locations for which few direct electrophysiological data are available, such as the ventrolateral medulla, the posterior hypothalamic nucleus, and the reuniens and paraventricular thalamic nuclei. Gentle mechanical stimulation was ineffective in inducing c-fos-like protein. The data suggest that c-fos can be used as a transynaptic marker for neuronal activity following noxious stimulation. However, c-fos is expressed only in some kinds of neurons following peripheral stimulation, and it therefore may be an incomplete marker for nociresponsive activity. In addition, at least a few neurons express c-fos protein in the absence of noxious stimulation. Experiments analyzing c-fos expression must be designed with care, as both extraneous stimuli and anesthetic depth influence the results.