Dietary fructans, but not cellulose, decrease triglyceride accumulation in the liver of obese Zucker fa/fa rats
Dietary fructans, but not cellulose, decrease triglyceride accumulation in the liver of obese Zucker fa/fa rats
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DOI：
10.1093/jn/132.5.967
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发表时间：
2002-05-01
影响因子：
4.2
通讯作者：
Delzenne, N
中科院分区：
文献类型：
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作者：
Daubioul, C;Rousseau, N;Delzenne, N
本研究的目的是比较饮食中补充不可消化的碳水化合物，不同的结肠细菌的发酵能力，对脂肪肝在不断增长的肥胖Zucker大鼠的影响。将雄性Zucker fa/fa大鼠分为三组：接受基础饮食的对照组、接受10 g高度发酵Synergy 1/100 g饮食的果聚糖组和接受10 g不良发酵Vivapur微晶纤维素/100 g饮食的纤维素组。消耗果聚糖的大鼠有一个较低的能量摄入量，较低的体重和较少的三酰甘油在肝脏中的积累，在体内评估的核磁共振（NMR）光谱，和离体生物化学和组织化学分析相比，控制和/或纤维素组。果聚糖的高发酵与纤维素相比，反映了更大的盲肠内容物，并由两倍更大的丙酸浓度在门静脉的大鼠相比，那些喂食果聚糖纤维素。通过测量从Zucker大鼠肝脏中分离的肝细胞从不同前体合成甘油三酯或总脂质的能力，我们表明，在用果聚糖处理的大鼠门静脉中测量的浓度下，丙酸盐选择性地减少乙酸盐掺入总脂质中，这一现象可能有助于沿着较低的能量摄入，在喂食果聚糖的肥胖Zucker大鼠的肝脏中甘油三酯积累较少。
This study was designed to compare the effects of dietary supplementation with nondigestible carbohydrates, differing in fermentability by colonic bacteria, on hepatic steatosis in growing obese Zucker rats. Male Zucker fa/fa rats were divided into three groups: a control group that received the basal diet, a fructan group that received 10 g highly fermented Synergy 1/100 g diet and a cellulose group that received 10 g poorly fermented Vivapur Microcrystalline cellulose/100 g diet. Rats consuming fructan had a lower energy intake, a lower body weight and less triacylglycerol accumulation in the liver as assessed in vivo by nuclear magnetic resonance (NMR) spectroscopy, and ex vivo by biochemical and histochemical analysis compared with the control and/or cellulose groups. The high fermentation of fructans compared with cellulose was reflected by greater cecal contents and by a twofold greater propionate concentration in the portal vein of rats fed fructan compared with those fed cellulose. By measuring the capacity of hepatocytes isolated from liver of Zucker rats to synthesize triglycerides or total lipids from different precursors, we showed that propionate, at the concentrations measured in the portal vein of rats treated with fructan, selectively decreased the incorporation of acetate into total lipids, a phenomenon that could contribute, along with the lower energy intake, to less triglyceride accumulation in the liver of obese Zucker rats fed dietary fructans.