Valuing museum specimens: high-throughput DNA sequencing on historical collections of New Guinea crowned pigeons (Goura)
Valuing museum specimens: high-throughput DNA sequencing on historical collections of New Guinea crowned pigeons (Goura)
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DOI：
10.1111/bij.12494
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发表时间：
2016-01-01
影响因子：
1.9
通讯作者：
Thebaud, Christophe
中科院分区：
文献类型：
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作者：
Besnard, Guillaume;Bertrand, Joris A. M.;Thebaud, Christophe
博物馆标本对于研究系统学和植物地理学以及生物多样性进化的其他方面特别重要。它们也是最近历史时期积累的标本的存放地，通常是研究最近灭绝或稀有物种的唯一途径。不幸的是，大多数博物馆标本产生低质量的DNA限制了它们在系统发育和种群遗传学研究中的广泛应用。测序技术的进步现在提供了分析这种材料的机会，即使是从非常小的组织中。在这里，我们应用Illumina技术（HiSeq）对三个冠鸽标本（Goura spp.）1879年至1934年间在新几内亚采集的鸟枪策略使我们能够快速可靠地组装完整的线粒体基因组和三个单拷贝基因系统发育标记的片段，即使双链DNA量很低（9至69 ng）。这些新序列的系统发育分配确认它们为古拉序列。因此，我们的研究证实了使用下一代测序方法来调查物种进化史的能力，这些物种的新鲜样本有限，但博物馆收藏品可用。这些方法大大增加了许多自然历史收藏品的遗传调查价值，特别是来自目前难以前景的生物多样性热点的收藏品。(C)2015年伦敦林奈学会，
Museum specimens are of particular importance for investigating systematics and the biogeography as well as other aspects of the evolution of biodiversity. They are also a depository of specimens accumulated over recent historical times and often the only way to study recently extinct or rare species. Unfortunately, most museum specimens yield low-quality DNA limiting their generalized use in phylogenetic and population genetic studies. Advances in sequencing technologies now offer opportunities to analyse such material even from very small tissue. Here, we applied the Illumina technology (HiSeq) on three specimens of crowned pigeon (Goura spp.) collected in New Guinea between 1879 and 1934. A shotgun strategy allowed us to quickly and reliably assemble complete mitochondrial genomes and fragments of three single-copy gene phylogenetic markers even with low double-stranded DNA quantity (9 to 69 ng). Phylogenetic assignments of these new sequences confirmed them as Goura sequences. Therefore, our study confirms the power of using next-generation sequencing methods to investigate the evolutionary history of species for which access to fresh samples is limited but museum collections are available. These approaches are considerably increasing the value of many natural history collections for genetic investigations, particularly from biodiversity hotspots that are presently difficult to prospect. (C) 2015 The Linnean Society of London,