Analysis of the cis-acting requirements for germ-line-specific splicing of the P-element ORF2-ORF3 intron.
Analysis of the cis-acting requirements for germ-line-specific splicing of the P-element ORF2-ORF3 intron.
复制标题
P 元件 ORF2-ORF3 内含子种系特异性剪接的顺式作用要求分析。
作者：
F. Laski;G. Rubin
P元件转座仅限于生殖系，因为该元件的第三内含子仅在生殖系细胞中剪接。我们发现，一个240 bp的片段，包含这个190 bp的内含子可以赋予生殖系特异性时，放置在另一个基因的背景下。我们发现，生殖系调控所需的顺式作用调控序列的地图附近，但不是在5'或3'剪接点。
P-element transposition is limited to the germ line because the element's third intron is only spliced in germ line cells. We show that a 240-bp fragment containing this 190-bp intron can confer germ line specificity when placed in the context of another gene. We find that the cis-acting regulatory sequences required for germ line regulation map near to, but not at, the 5' or 3' splice junctions.